Center of Environmental Science and Engineering, College of Environment and Resource, Shanxi University, Taiyuan, Shanxi 030006, PR China.
J Hazard Mater. 2010 Feb 15;174(1-3):54-8. doi: 10.1016/j.jhazmat.2009.09.015. Epub 2009 Sep 10.
In the present study, protein oxidation (PCO content) and DNA-protein crosslinks (DPC coefficient) were investigated in the viscera of mice exposed to a municipal landfill leachate at various concentrations for 7 days. The study was designed to investigate the injuries and a possible mechanism of landfill leachate-induced toxicity on mammals. The results indicate that the leachate sample changed the ratio of viscera to body weight in all organs tested, and the effect on the brain was more obvious than that on other organs. The leachate ingestion increased PCO levels in the brain, kidney, liver and spleen in a concentration-dependent manner, but did not affect the content in the heart. Also, the leachate sample enhanced DPC formation in the tested organs in a concentration-dependent manner, and the responses of the liver, kidney and spleen were more sensitive than that of the brain and heart. These findings provide further evidence that landfill leachate-induced toxicity on mammals might involve the formation of free radicals, either via autoxidation or by enzyme-catalyzed oxidation of pollutants in leachate, and then attack of proteins and nucleic acids via generated free radicals.
在本研究中，研究了暴露于不同浓度的城市垃圾渗滤液中的小鼠内脏中的蛋白质氧化（PCO 含量）和 DNA-蛋白质交联（DPC 系数）。本研究旨在研究垃圾渗滤液对哺乳动物的损伤及其可能的毒性机制。结果表明，渗滤液样品改变了所有测试器官的内脏与体重的比例，对大脑的影响比其他器官更明显。渗滤液摄入使大脑、肾脏、肝脏和脾脏中的 PCO 水平呈浓度依赖性增加，但对心脏中的含量没有影响。此外，渗滤液样品以浓度依赖的方式增强了测试器官中的 DPC 形成，肝脏、肾脏和脾脏的反应比大脑和心脏更敏感。这些发现进一步证明，垃圾渗滤液对哺乳动物的毒性可能涉及自由基的形成，自由基可以通过渗滤液中污染物的自动氧化或酶促氧化产生，然后通过生成的自由基攻击蛋白质和核酸。
