College of Marine Science, Shandong University at Weihai, Weihai 264209, PR China.
School of Life Sciences, Heriot-Watt University, Riccarton, Edinburgh EH14 4AS, UK.
Int J Syst Evol Microbiol. 2010 Aug;60(Pt 8):1944-1947. doi: 10.1099/ijs.0.018523-0. Epub 2009 Sep 25.
A taxonomic study was performed on strain D7015T, which was isolated from coastal sediment close to a coal-fired power station in Qingdao, China. Cells of strain D7015T were Gram-positive, non-motile, diphtheroid rods that grew in the presence of 0-8% (w/v) NaCl and at 4-37 degrees C, with optimum growth at 1% (w/v) NaCl and 30-32 degrees C. The DNA G+C content was 65.0 mol%. The major fatty acids were C18:1omega9c (56.18%), C16:0 (38.02%), C16:1omega7c (4.45%), C18:0 (1.0%) and C14:0 (0.35%). On the basis of morphological, physiological and phylogenetic characteristics, strain D7015T was classified in the genus Corynebacterium. It exhibited a 16S rRNA gene sequence similarity of 95.9% and a DNA-DNA relatedness value of 20.4% with Corynebacterium halotolerans DSM 44683T. Strain D7015T was sufficiently different from recognized species of the genus Corynebacterium to be considered to represent a novel species. The name Corynebacterium marinum sp. nov. is proposed, with strain D7015T (=CGMCC 1.6998T=NRRL B-24779T) as the type strain.
对分离自中国青岛一燃煤电厂附近沿海沉积物的菌株 D7015T 进行了分类学研究。菌株 D7015T 的细胞呈革兰阳性、非运动性、类白喉棒状,可在 0-8%(w/v)NaCl 浓度和 4-37°C 的条件下生长,最佳生长条件为 1%(w/v)NaCl 和 30-32°C。其 DNA G+C 含量为 65.0mol%。主要脂肪酸为 C18:1ω9c(56.18%)、C16:0(38.02%)、C16:1ω7c(4.45%)、C18:0(1.0%)和 C14:0(0.35%)。根据形态学、生理学和系统发育特征,菌株 D7015T 被归入棒状杆菌属。其 16S rRNA 基因序列相似性为 95.9%,与耐盐棒状杆菌 DSM 44683T 的 DNA-DNA 同源性值为 20.4%。菌株 D7015T 与公认的棒状杆菌属物种有足够的差异,被认为代表了一个新的物种。建议将其命名为海洋棒状杆菌(Corynebacterium marinum)sp. nov.,其模式菌株为 D7015T(=CGMCC 1.6998T=NRRL B-24779T)。