Jani Kunal, Khare Kaustubh, Senik Svetlana, Karodi Prachi, Vemuluri Venkata Ramana, Bandal Jayashree, Shouche Yogesh, Rale Vinay, Sharma Avinash
National Centre for Microbial Resource, National Centre for Cell Science, Pune, Maharashtra 411007, India.
Symbiosis School of Biological Sciences, Symbiosis International University, Pune, Maharashtra 412115, India.
Int J Syst Evol Microbiol. 2018 Jan;68(1):241-247. doi: 10.1099/ijsem.0.002491. Epub 2017 Nov 17.
A Gram-stain-positive, rod-shaped, non-motile bacterium, strain PRD07, was isolated from Godavari river, India during the world's largest spiritual and religious mass bathing event 'Kumbh Mela'. Molecular analysis using 16S rRNA gene sequencing and phylogenetic analysis reveals the distinct phylogenetic positioning of strain PRD07 within the genus Corynebacterium. The strain demonstrated highest sequence similarity to Corynebacterium imitans DSM 44264 (97.9 %), Corynebacterium appendicis DSM 44531 (97.1 %) and <96.7 % with all other members of the genus Corynebacterium. The G+C content of PRD07 was 68.5 mol% (Tm) and the DNA-DNA hybridization depicts 61.09 % genomic relatedness with C. imitans DSM 44264. Chemotaxonomic assessment of strain PRD07 suggested presence of C16 : 0 (31.6 %), C18 : 0 (3.5 %) and C18 : 1ω9c (58.6 %) as the major cellular fatty acids. The major polar lipids of strain PRD07 were phosphatidylglycerol, diphosphatidylglycerol and glycophospholipid. Differentiating molecular, phylogenetic and chemotaxonomic characteristics of strain PRD07 with its closest relatives necessitated the description of strain PRD07 as a novel species of genus Corynebacterium for which the name Corynebacteriumgodavarianum sp. nov., has been proposed. The type strain is PRD07 (=MCC 3388=KCTC 39803=LMG 29598).
一株革兰氏阳性、杆状、无运动性的细菌PRD07，是在世界上最大的精神和宗教集体沐浴活动“大壶节”期间从印度戈达瓦里河中分离出来的。使用16S rRNA基因测序的分子分析和系统发育分析揭示了菌株PRD07在棒状杆菌属内独特的系统发育定位。该菌株与模仿棒状杆菌DSM 44264（97.9%）、阑尾棒状杆菌DSM 44531（97.1%）表现出最高的序列相似性，与棒状杆菌属的所有其他成员的相似性均<96.7%。PRD07的G+C含量为68.5 mol%（熔解温度），DNA-DNA杂交显示与模仿棒状杆菌DSM 44264的基因组相关性为61.09%。对菌株PRD07的化学分类学评估表明，主要细胞脂肪酸为C16 : 0（31.6%）、C18 : 0（3.5%）和C18 : 1ω9c（58.6%）。菌株PRD07的主要极性脂类为磷脂酰甘油、双磷脂酰甘油和糖磷脂。菌株PRD07与其最亲近的亲缘种在分子、系统发育和化学分类学特征上的差异，使得有必要将菌株PRD07描述为棒状杆菌属的一个新物种，为此提出了“戈达瓦里棒状杆菌”（Corynebacteriumgodavarianum sp. nov.）这一名称。模式菌株为PRD07（=MCC 3388=KCTC 39803=LMG 29598）。
