Kushneria sinocarnis sp. nov., a moderately halophilic bacterium isolated from a Chinese traditional cured meat.

A Gram-negative, aerobic, moderately halophilic bacterium, designated strain Z35T, was isolated from a Chinese traditional cured meat produced in Wuhan. The isolate grew with 1-20% NaCl (optimum 10%), at 4-42 degrees C (optimum 37 degrees C) and at pH 4.5-8.5 (optimum pH 7.0). Cells of strain Z35T were not motile and were rod or oval shaped. The genomic DNA G+C content was 59.1 mol%. The isoprenoid quinones were Q-9 (88.96%), Q-8 (9.46%) and Q-10 (1.58%). The major fatty acids were C19:0 cyclo omega8c, C16:0 and C17:0 cyclo. Phylogenetic analysis based on 16S rRNA gene sequence analysis indicated that strain Z35T was closely related to the type strains of Kushneria species with 93.7-95.3% sequence similarities. Phylogenetic analysis based on 23S rRNA gene sequence similarity values also confirmed the phylogenetic position of the isolate. Therefore, based on the phenotypic, chemotaxonomic and phylogenetic evidence, strain Z35T is affiliated to Kushneria, but is clearly differentiated from other species of this genus and represents a new member, for which the name Kushneria sinocarnis sp. nov. is proposed. The type strain is Z35T (=CCTCC AB 209027T =NRRL B-59197T =DSM 23229T).