Yun Ji-Hyun, Park Seong-Kyu, Lee June-Young, Jung Mi-Ja, Bae Jin-Woo
Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Sep;67(9):3576-3582. doi: 10.1099/ijsem.0.002170. Epub 2017 Sep 4.
A halophilic bacterial strain, X49T, was isolated from the Korean traditional salt-fermented seafood Daemi-jeot. X49T was an obligately aerobic, Gram-stain-negative, motile, oval or rod-shaped (0.5-1.0×1.2-3.2 µm) bacterium. After 2 days of growth, colonies on Marine agar medium were orange and circular with entire margins. X49T growth was detected at 10-37 °C and pH 4.5-8.5 in the presence of 0-26 % (w/v) NaCl. The 16S rRNA gene sequence of strain X49T was most similar to that of the type strain of Kushneria marisflavi SW32T and shared a sequence similarity of 94.7-98.6 % with type strains of species of the genus Kushneria. The predominant fatty acids were C16 : 0, C18 : 1ω7c and C12 : 0 3OH. The major isoprenoid quinone was Q9 (93 %), and minor quinones were Q8 (4 %) and Q10 (3 %). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylserine, two unidentified aminolipids, two unidentified phospholipids and two unidentified lipids. The genomic DNA G+C content was 59.1 mol%. The level of the ANI value between strain X49T and K. marisflavi SW32T, the most closely related species of the genus Kushneria, was 89.32 %. Based on the low ANI value, strain X49T and its reference strains represent genotypically distinct species. Based on this polyphasic taxonomic analysis, strain X49T represents a novel species of the genus Kushneria. The name Kushneria konosiri sp. nov. is proposed and the type strain is X49T (=KACC 14623T=JCM 16805T).
从韩国传统盐渍发酵海鲜大酱渍物中分离出一株嗜盐细菌菌株X49T。X49T是一种专性需氧、革兰氏染色阴性、具运动性、椭圆形或杆状（0.5 - 1.0×1.2 - 3.2 µm）的细菌。在海洋琼脂培养基上生长2天后，菌落呈橙色，圆形，边缘整齐。在0 - 26%（w/v）NaCl存在的情况下，X49T在10 - 37°C和pH 4.5 - 8.5条件下可生长。菌株X49T的16S rRNA基因序列与海黄库什纳氏菌SW32T模式菌株的序列最为相似，与库什纳氏菌属其他种的模式菌株序列相似性为94.7 - 98.6%。主要脂肪酸为C16 : 0、C18 : 1ω7c和C12 : 0 3OH。主要类异戊二烯醌为Q9（93%），次要醌为Q8（4%）和Q10（3%）。极性脂为双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺和磷脂酰丝氨酸，两种未鉴定的氨基脂，两种未鉴定的磷脂和两种未鉴定的脂。基因组DNA G + C含量为59.1 mol%。菌株X49T与库什纳氏菌属中亲缘关系最近的海黄库什纳氏菌SW32T之间的ANI值为89.32%。基于较低的ANI值，菌株X49T及其参考菌株代表基因型不同的物种。基于此多相分类分析，菌株X49T代表库什纳氏菌属的一个新物种。提议将其命名为海黄库什纳氏菌（Kushneria konosiri sp. nov.），模式菌株为X49T（=KACC 14623T=JCM 16805T）。
