Department of Entomology and Plant Pathology, Mississippi State University, Mississippi State, MS 39762, USA.
Arch Virol. 2009;154(11):1729-35. doi: 10.1007/s00705-009-0510-x. Epub 2009 Sep 26.
An undescribed virus with isometric particles and a diameter of ca. 30 nm was identified in diseased samples of wild and cultivated Rubus species and molecularly characterized. Its genome was 6,463 nt, excluding the 3'-terminal poly(A) tail, and contained a single open reading frame coding for a 2,035-amino-acid-long precursor polypeptide (p223). The amino terminal portion of p223, identified as a replication-associated polyprotein, contained conserved motifs of methyltransferase, endopeptidase/protease, helicase and RNA-dependent RNA polymerase. The carboxy terminus of the large polypeptide is involved in the formation of two viral coat protein subunits with deduced molecular masses of 23 and 21 kDa. Pairwise comparisons and phylogenetic analyses showed closest relationships of this virus with oat blue dwarf virus and citrus sudden death-associated virus, sharing levels of genome sequence conservation far below the species demarcation level established for tymovirids. Our data indicate that this virus, for which the name blackberry virus S (BlVS) is proposed, is a hitherto undescribed species of the genus Marafivirus, family Tymoviridae. A survey conducted in Mississippi, USA, has shown that BlVS is also present in cultivated Rubus germplasm. This work represents the first report of a marafivirus infecting small fruits.
一种具有等径颗粒和直径约 30nm 的未描述病毒在野生和栽培悬钩子属植物的患病样本中被鉴定出来，并进行了分子特征分析。其基因组为 6463nt，不包括 3'末端的 poly(A)尾，包含一个单一的开放阅读框，编码一个 2035 个氨基酸的前体多肽（p223）。p223 的氨基末端部分被鉴定为复制相关的多蛋白，含有甲基转移酶、内切酶/蛋白酶、解旋酶和 RNA 依赖的 RNA 聚合酶的保守基序。大多肽的羧基末端参与形成两个病毒外壳蛋白亚基，推测分子量分别为 23 和 21kDa。成对比较和系统发育分析表明，该病毒与燕麦蓝矮病毒和柑橘猝死相关病毒关系最密切，其基因组序列保守程度远低于 tymovirids 种间划分的水平。我们的数据表明，这种病毒，我们提议将其命名为黑莓病毒 S（BlVS），是迄今为止未被描述的 Marafivirus 属病毒，属于 Tymoviridae 科。在美国密西西比州进行的一项调查表明，BlVS 也存在于栽培的悬钩子属植物种质中。这项工作代表了第一个感染小水果的 marafivirus 的报告。
