一步法测定人尿中卡那霉素：尿素增强纳米金颗粒表面等离子体共振光散射法

Determination of human urinary kanamycin in one step using urea-enhanced surface plasmon resonance light-scattering of gold nanoparticles.

机构信息

The Key Laboratory of Bioorganic Chemistry and Molecular Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.

出版信息

Anal Bioanal Chem. 2009 Dec;395(7):2397-403. doi: 10.1007/s00216-009-3134-9. Epub 2009 Sep 27.

DOI:10.1007/s00216-009-3134-9

PMID:19784833

Abstract

The purpose of this study was to establish a simple, sensitive analytical method for kanamycin (KANA) in human urine. Enhancement of the plasmon resonance light-scattering (PRLS) of gold nanoparticles (AuNPs) by KANA provided the basis for this analytical method. At pH 6.7, KANA induced AuNPs aggregation with enhanced PRLS. The PRLS of the AuNPs-KANA system was further enhanced by addition of urea. The linear range and detection limit for KANA were from 20-800 nmol L(-1) and 2 nmol L(-1), respectively. Potential interfering substances present in urine had a negligible effect on the determination, thus preliminary sample separations were not necessary. Recovery of KANA from spiked human urine was 94-104%. This simple, sensitive method, using urea to enhance the PRLS of the AuNPs-KANA system, may provide a new approach for determination of compounds rich in OH groups.

摘要

本研究旨在建立一种简单、灵敏的分析方法，用于检测人尿中的卡那霉素（KANA）。KANA 增强金纳米粒子（AuNPs）的等离子体共振光散射（PRLS）为该分析方法提供了基础。在 pH 6.7 下，KANA 诱导 AuNPs 聚集，增强了 PRLS。加入尿素进一步增强了 AuNPs-KANA 体系的 PRLS。KANA 的线性范围和检测限分别为 20-800 nmol L(-1)和 2 nmol L(-1)。尿液中存在的潜在干扰物质对测定的影响可以忽略不计，因此不需要进行初步的样品分离。从加标人尿中回收 KANA 的回收率为 94-104%。该方法简单、灵敏，利用尿素增强 AuNPs-KANA 体系的 PRLS，可能为测定富含 OH 基团的化合物提供一种新方法。