Department of Physiology and Biophysics, Center for Biomembrane Systems, University of California, Irvine, California 92697-4560, USA.
Protein Sci. 2009 Dec;18(12):2624-8. doi: 10.1002/pro.256.
Hydropathy plot methods form a cornerstone of membrane protein research, especially in the early stages of biochemical and structural characterization. Membrane Protein Explorer (MPEx), described in this article, is a refined and versatile hydropathy-plot software tool for analyzing membrane protein sequences. MPEx is highly interactive and facilitates the characterization and identification of favorable protein transmembrane regions using experiment-based physical and biological hydrophobicity scales. Besides allowing the consequences of sequence mutations to be examined, it provides tools for aiding the design of membrane-active peptides. MPEx is freely available as a Java Web Start application from our web site at http://blanco.biomol.uci.edu/mpex.
疏水性绘图方法是膜蛋白研究的基石，特别是在生化和结构特征描述的早期阶段。本文介绍的膜蛋白探索者（MPEx）是一种经过改进的、多功能的疏水性绘图软件工具，用于分析膜蛋白序列。MPEx 具有高度的交互性，使用基于实验的物理和生物疏水性尺度，有助于对有利的蛋白质跨膜区域进行特征描述和鉴定。除了允许检查序列突变的后果外，它还提供了辅助设计膜活性肽的工具。MPEx 可作为 Java Web Start 应用程序从我们的网站 http://blanco.biomol.uci.edu/mpex 免费获得。
