多药外排转运蛋白 CmeABC 对不同种弯曲菌属抗生素耐药性的贡献。
Contribution of the multidrug efflux transporter CmeABC to antibiotic resistance in different Campylobacter species.
机构信息
Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, Iowa 50011, USA.
出版信息
Foodborne Pathog Dis. 2010 Jan;7(1):77-83. doi: 10.1089/fpd.2009.0354.
Abstract
CmeABC, a multidrug efflux system in Campylobacter jejuni, plays an important role in the resistance to different antimicrobials and toxic compounds. Although this efflux system has been well characterized in C. jejuni and to a less extent in C. coli, it is unknown if CmeABC homologs are functional in other Campylobacter spp. In this study, the cmeABC homologs were identified and functionally characterized in five Campylobacter species including C. jejuni, C. coli, C. lari, C. upsaliensis, and C. fetus. Our results indicated that cmeABC is present in all five Campylobacter spp. and the genomic organization of this efflux operon is similar among the Campylobacter spp. Insertional mutagenesis of cmeB increased the susceptibilities of all the five Campylobacter spp. to structurally diverse antimicrobials. Together, these results indicated that the CmeABC efflux system is conserved at both the genomic and functional levels in all five Campylobacter spp. examined in this study, further highlighting the significant role of CmeABC in Campylobacter pathobiology.
摘要
空肠弯曲菌中的 CmeABC 是一种多药外排系统，在抵抗不同抗菌药物和有毒化合物方面发挥着重要作用。尽管该外排系统在空肠弯曲菌中以及在较小程度上在大肠弯曲菌中得到了很好的表征，但其他弯曲菌属中 CmeABC 同源物是否具有功能尚不清楚。在这项研究中，鉴定并功能表征了五种弯曲菌属包括空肠弯曲菌、大肠弯曲菌、副弯弯曲菌、乌斯宾斯基弯曲菌和胎儿弯曲菌中的 cmeABC 同源物。我们的结果表明 cmeABC 存在于所有五种弯曲菌属中，并且该外排操纵子的基因组组织在弯曲菌属中相似。cmeB 的插入突变增加了所有五种弯曲菌属对抗生素的敏感性。总之，这些结果表明，在本研究中检测的所有五种弯曲菌属中，CmeABC 外排系统在基因组和功能水平上都是保守的，进一步强调了 CmeABC 在弯曲菌发病机制中的重要作用。
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