Laboratório de Doenças Parasitárias, Departamento de Saúde Coletiva Veterinária e Saúde Pública, Faculdade de Medicina Veterinária, Universidade Federal Fluminense, Niterói, RJ, Brasil.
Braz J Med Biol Res. 2009 Oct;42(10):918-20. doi: 10.1590/s0100-879x2009005000023. Epub 2009 Sep 4.
Human infection with fish parasites can result from the ingestion of incompletely cooked or raw fish, giving origin to parasitic diseases such as anisakiasis, caused by parasites of the Anisakidae family. The present study assessed the in vitro larvicidal effect of two monoterpene compounds, geraniol and citronellal, against Contracaecum sp (Nematoda: Anisakidae). Four hundred live larvae of Contracaecum sp obtained from 'traíra' fish (Hoplias malabaricus, Bloch, 1974) were analyzed on 40 Petri dishes (10 larvae each) with the compounds to be tested. The final concentrations tested for each compound were 250, 125, 62.5, and 31.2 microg/mL and the evaluation was carried out at five different times (2, 4, 8, 24, and 48 h). The larvicidal action of geraniol and citronellal was statistically superior (P < 0.005) to the control (1% ethanol) at concentrations of 250 and 31.2 microg/mL (geraniol) and 250, 125, and 62.5 microg/mL (citronellal). However, no larvicidal activity was observed at concentrations of 125 and 62.5 microg/mL for geraniol and 31.2 microg/mL for citronellal. When the larvicidal action of geraniol was compared to that of citronellal, the former was found to be statistically superior (P < 0.05) to the latter at concentrations of 250 and 31.2 microg/mL. On the other hand, citronellal was statistically superior (P < 0.005) to geraniol at concentrations of 125 and 62.5 microg/mL. The larval mortality rate in terms of time (hours) was higher for geraniol with the passing of time at the 250 microg/mL concentration. At this concentration (in 48 h) the best larvicidal effect was observed with 90% lethality. The larvae were considered to be dead using no motility and loss of structural integrity as parameters. The data indicate that natural terpene compounds should be more explored for antihelminthic activity and can be useful for other studies about anisakiasis treatment.
人类感染鱼类寄生虫可因食用未煮熟或生的鱼类而引起，导致寄生虫病，如异尖线虫病，由异尖科寄生虫引起。本研究评估了两种单萜化合物香叶醇和香茅醛对 Contracaecum sp（线虫：异尖科）的体外幼虫杀幼虫作用。从“traíra”鱼（Hoplias malabaricus，Bloch，1974）中获得了 400 条活 Contracaecum sp 幼虫，在 40 个培养皿中（每个培养皿 10 条幼虫）用待测试的化合物进行分析。每种化合物的最终测试浓度为 250、125、62.5 和 31.2μg/mL，并在五个不同时间（2、4、8、24 和 48 h）进行评估。香叶醇和香茅醛的杀幼虫作用明显优于对照（1%乙醇）（P < 0.005），浓度为 250 和 31.2μg/mL（香叶醇）和 250、125 和 62.5μg/mL（香茅醛）。然而，香叶醇的浓度为 125 和 62.5μg/mL，香茅醛的浓度为 31.2μg/mL 时，未观察到杀幼虫活性。当香叶醇的杀幼虫作用与香茅醛的杀幼虫作用进行比较时，发现前者在浓度为 250 和 31.2μg/mL 时明显优于后者（P < 0.05）。另一方面，香茅醛在浓度为 125 和 62.5μg/mL 时明显优于香叶醇（P < 0.005）。随着时间的推移，250μg/mL 浓度下，香叶醇的幼虫死亡率更高。在该浓度（48 h）下，观察到 90%的致死率，表现出最好的杀幼虫效果。幼虫被认为是死的，没有运动和结构完整性的损失作为参数。数据表明，天然萜类化合物应该更多地用于抗蠕虫活性的研究，并且可以用于其他关于异尖线虫病治疗的研究。
