Zhao Shu-Min, Li Cheng-Tao, Zhang Su-Hua, Li Li, Lin Yuan, Que Ting-Zhi
Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, Shanghai 200063, China.
Fa Yi Xue Za Zhi. 2009 Aug;25(4):267-70.
To establish and evaluate the method of full sibling identification based on the number of allele shared among autosomal STR Loci.
Two hundred and eighty full sibling pairs and 2,003 unrelated individual pairs were genotyped in 15 STR loci with Identifiler Kit, and the number of allele shared among the 15 STR loci (S15) and full sibling index (FSI) were calculated. Fisher discriminant functions were established with SAS 8.2 software based on S15, the power of which were compared with ITO method.
The distribution of S15, in full sibling pair group and unrelated individual pair group were in accord with normal distribution. The established Fisher discriminant functions for each group were Z(FS)= 3.26970S15-31.51174 and Z(UI)=1.70058S15-8.524 11, respectively. The average error of probability in sibling and unrelated pair group was 0.0298. There was no statistically significant difference on the power of full sibling discriminant between the method based on the number of allele shared among the 15 STR loci or the CODIS 13 STR loci and the ITO method.
The method based on the number of allele shared among the 15 STR loci in full sibling identification is convenient, credible, easy to handling and unaffected by the allele frequency of STR loci.
建立并评估基于常染色体STR基因座间共享等位基因数目的全同胞鉴定方法。
应用Identifiler试剂盒对280对全同胞及2003对无关个体进行15个STR基因座的基因分型，计算15个STR基因座间共享等位基因数（S15）及全同胞指数（FSI）。基于S15，用SAS 8.2软件建立Fisher判别函数，并与ITO法比较其效能。
全同胞组与无关个体组S15的分布均符合正态分布。建立的各组Fisher判别函数分别为Z(FS)= 3.26970S15-31.51174和Z(UI)=1.70058S15-8.524 11。同胞组与无关个体组概率平均误差为0.0298。基于15个STR基因座或CODIS 13个STR基因座共享等位基因数目的方法与ITO法在全同胞判别效能上无统计学差异。
基于15个STR基因座共享等位基因数目的全同胞鉴定方法简便、可靠、易操作，且不受STR基因座等位基因频率影响。
