Shpitzer T, Hamzany Y, Bahar G, Feinmesser R, Savulescu D, Borovoi I, Gavish M, Nagler R M
Department of Otorhinolaryngology, Rabin Medical Center, Petah Tiqva and Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
Br J Cancer. 2009 Oct 6;101(7):1194-8. doi: 10.1038/sj.bjc.6605290.
Oral cancer is a common and lethal malignancy. Direct contact between saliva and the oral cancer lesion makes measurement of tumour markers in saliva an attractive alternative to serum testing.
We tested 19 tongue cancer patients, measuring the levels of 8 salivary markers related to oxidative stress, DNA repair, carcinogenesis, metastasis and cellular proliferation and death.
Five markers increased in cancer patients by 39-246%: carbonyls, lactate dehydrogenase, metalloproteinase-9 (MMP-9), Ki67 and Cyclin D1 (CycD1) (P< or =0.01). Three markers decreased by 16-29%: 8-oxoguanine DNA glycosylase, phosphorylated-Src and mammary serine protease inhibitor (Maspin) (P< or =0.01). Increase in salivary carbonyls was profound (by 246%, P=0.012); alterations in CycD1 (87% increase, P=0.000006) and Maspin (29% decrease, P=0.007) were especially significant. Sensitivity values of these eight analysed markers ranged from 58% to 100%; specificity values ranged from 42% to 100%. Both values were especially high for the CycD1 and Maspin markers, 100% for each value of each marker. These were also high for carbonyls, 90% and 80%, respectively, and for MMP-9, 100% and 79%, respectively.
The significance of each salivary alteration is discussed. As all alterations correlated with each other, they may belong to a single carcinogenetic network. Cancer-related changes in salivary tumour markers may be used as a diagnostic tool for diagnosis, prognosis and post-operative monitoring.
口腔癌是一种常见的致命恶性肿瘤。唾液与口腔癌病变的直接接触使得唾液中肿瘤标志物的检测成为血清检测的一种有吸引力的替代方法。
我们对19名舌癌患者进行了检测，测量了8种与氧化应激、DNA修复、致癌作用、转移以及细胞增殖和死亡相关的唾液标志物的水平。
5种标志物在癌症患者中升高了39%至246%：羰基、乳酸脱氢酶、金属蛋白酶-9（MMP-9）、Ki67和细胞周期蛋白D1（CycD1）（P≤0.01）。3种标志物降低了16%至29%：8-氧代鸟嘌呤DNA糖基化酶、磷酸化-Src和乳腺丝氨酸蛋白酶抑制剂（Maspin）（P≤0.01）。唾液羰基的升高幅度很大（升高246%，P = 0.012）；CycD1（升高87%，P = 0.000006）和Maspin（降低29%，P = 0.007）的变化尤为显著。这8种分析标志物的敏感性值范围为58%至100%；特异性值范围为42%至100%。CycD1和Maspin标志物的这两个值都特别高，每个标志物的每个值均为100%。羰基的这两个值也很高，分别为90%和80%，MMP-9的分别为100%和79%。
讨论了每种唾液变化的意义。由于所有变化相互关联，它们可能属于一个单一的致癌网络。唾液肿瘤标志物中与癌症相关的变化可作为诊断、预后和术后监测的诊断工具。
