Laboratory of Medicinal Chemistry, Center for Cancer Research, National Cancer Institute/NIH, 376 Boyles Street, Frederick, MD 21702, USA.
Curr Opin Chem Biol. 2009 Oct;13(4):406-13. doi: 10.1016/j.cbpa.2009.06.021. Epub 2009 Jul 20.
Carbohydrate arrays, also referred to as glycan arrays, are composed of various oligosaccharides and/or polysaccharides immobilized on a solid support in a spatially defined arrangement. This technology provides a powerful, high-throughput approach to examining carbohydrate-macromolecule interactions, and glycan arrays have had a significant impact on the field of glycobiology. This review focuses on recent advances in glycan array technology, limitations, and opportunities for improvement. In particular, new methods for the production of natural glycan arrays and chemoenzymatic approaches are greatly expanding the diversity of structures on arrays. Since multivalent complex formation is generally required to achieve tight binding, methods to evaluate and modulate presentation are vital for enhancing the capabilities of this technology.
碳水化合物微阵列，也称为糖基化微阵列，由固定在固体支持物上的各种寡糖和/或多糖组成，在空间上呈特定排列。这项技术为研究碳水化合物-大分子相互作用提供了一种强大的高通量方法，糖基化微阵列对糖生物学领域产生了重大影响。本综述重点介绍了糖基化微阵列技术的最新进展、局限性和改进机会。特别是，用于生产天然糖基化微阵列的新方法和化学酶方法极大地扩展了微阵列上结构的多样性。由于多价复杂形成通常是实现紧密结合所必需的，因此评估和调节呈现的方法对于增强该技术的能力至关重要。
