College of Chemistry, Chongqing Normal University, Chongqing, 401331, China.
Curr Med Chem. 2014;21(3):288-95. doi: 10.2174/09298673113206660267.
Carbohydrate arrays are used as high-throughput screening platforms to study the carbohydrate-mediated recognition events for glycobiology. The polysaccharide arrays are easy to fabricate by non-covalently or covalently immobilizing polysaccharides onto array surfaces because polysaccharides have hydrophobic interactions. Oligosaccharides must be derived and covalently or non-covalently immobilized onto array surfaces to fabricate oligosaccharide arrays because they have hydrophilic interactions. At the moment, carbohydrate arrays are mainly used to study the carbohydrate-protein interactions and carbohydrate-binding lectins or antibodies, which are possible to be applied to clinics and diagnoses. This review mainly summed up the new fabrication strategies of carbohydrate arrays and their applications in recent four years.
碳水化合物微阵列被用作高通量筛选平台，以研究糖生物学中碳水化合物介导的识别事件。多糖微阵列可以通过非共价或共价固定多糖到阵列表面来轻松制备，因为多糖具有疏水相互作用。寡糖必须衍生并共价或非共价固定到阵列表面，以制备寡糖微阵列，因为它们具有亲水相互作用。目前，碳水化合物微阵列主要用于研究碳水化合物-蛋白质相互作用以及可能应用于临床和诊断的碳水化合物结合凝集素或抗体。本综述主要总结了近年来碳水化合物微阵列的新制备策略及其应用。
