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Evidence for salt-associated restriction pattern modifications in the archaeobacterium Haloferax mediterranei.

作者信息

Juez G, Rodriguez-Valera F, Herrero N, Mojica F J

机构信息

Departamento de Genética Molecular y Microbiología, Universidad de Alicante, Spain.

出版信息

J Bacteriol. 1990 Dec;172(12):7278-81. doi: 10.1128/jb.172.12.7278-7281.1990.

DOI:10.1128/jb.172.12.7278-7281.1990
PMID:1979325
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC210857/
Abstract

DNA restriction pattern modifications were detected when Haloferax mediterranei was grown in low (10%) salt concentrations. After cells were grown again in optimal (25%) salt concentrations, the original pattern was recovered. These salt-associated DNA modifications were revealed with 5% of the 160 DNA fragments cloned and used as probes in hybridization experiments. Patterns obtained when genomic DNA was digested with different restriction enzymes showed that these modifications are related not to insertions or deletions in genome but to modifications of some specific sequences.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b1e2/210857/4d95f624aaa5/jbacter00166-0681-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b1e2/210857/2d2f67e3a4c9/jbacter00166-0681-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b1e2/210857/9a8bb282d1a8/jbacter00166-0681-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b1e2/210857/4d95f624aaa5/jbacter00166-0681-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b1e2/210857/2d2f67e3a4c9/jbacter00166-0681-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b1e2/210857/9a8bb282d1a8/jbacter00166-0681-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b1e2/210857/4d95f624aaa5/jbacter00166-0681-c.jpg

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本文引用的文献

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High-frequency mutations in a plasmid-encoded gas vesicle gene in Halobacterium halobium.在嗜盐菌的质粒编码的气液胞基因中高频突变。
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Unusual physical organization of the Halobacterium genome.嗜盐菌基因组异常的物理结构。
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Characterization of a halobacterial gene affecting bacterio-opsin gene expression.
Nucleic Acids Res. 1984 Oct 25;12(20):7949-59. doi: 10.1093/nar/12.20.7949.
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Evidence for two restriction-modification systems in Halobacterium cutirubrum.盐红嗜盐菌中两种限制修饰系统的证据。
J Bacteriol. 1985 Aug;163(2):783-4. doi: 10.1128/jb.163.2.783-784.1985.