Varga B, Migliardo F, Takacs E, Vertessy B, Magazù Salvatore, Telling M T F
J Biol Phys. 2010 Mar;36(2):207-20. doi: 10.1007/s10867-009-9177-5. Epub 2009 Oct 1.
The present work aims to characterize the dynamical behavior of proteins immersed in bio-preserving liquids and glasses. For this purpose, the protein dUTPase was chosen, while the selected solvents were glycerol, a triol, and some homologous disaccharides, i.e., trehalose, maltose, and sucrose, which are known to be very effective bio-preserving agents. The results highlight that the disaccharides show a slowing down effect on the water dynamics, which is stronger for trehalose than in the case of the other disaccharides. Furthermore, a characterization of the medium which hosts the protein is performed by using an operative definition of fragility based on the mean square displacement extracted by elastic incoherent neutron scattering, which is directly connected to Angell's kinetic fragility based on the viscosity. Finally, a study of the dynamics of the protein sequestered within the solvents is performed. The result shows that the protein dynamics is coupled with that of the surrounding matrix.
本研究旨在表征浸入生物保存液和玻璃中的蛋白质的动力学行为。为此，选择了蛋白质dUTPase，而选定的溶剂是甘油（一种三元醇）和一些同源二糖，即海藻糖、麦芽糖和蔗糖，它们已知是非常有效的生物保存剂。结果表明，二糖对水动力学有减缓作用，海藻糖的这种作用比其他二糖更强。此外，通过基于弹性非相干中子散射提取的均方位移对脆性进行操作定义，来对容纳蛋白质的介质进行表征，该均方位移与基于粘度的安吉尔动力学脆性直接相关。最后，对溶剂中隔离的蛋白质的动力学进行了研究。结果表明，蛋白质动力学与周围基质的动力学相互耦合。
