Jarjour W, Mizzen L A, Welch W J, Denning S, Shaw M, Mimura T, Haynes B F, Winfield J B
Division of Rheumatology and Immunology, University of North Carolina, Chapel Hill 27514.
J Exp Med. 1990 Dec 1;172(6):1857-60. doi: 10.1084/jem.172.6.1857.
Rabbit antibodies to hsp58 (P1), the human homologue of the Escherichia coli stress protein groEL, react specifically in indirect immunofluorescence and complement-dependent microcytoxicity experiments with a cell surface antigen expressed constitutively by T cell lines bearing gamma/delta receptors. This anti-hsp58-reactive antigen is not demonstrable on T cells that express alpha/beta receptors or on various cells that lack T cell receptors. Certain evidence was obtained to suggest that the target antigen on the surface of gamma/delta T cells is a approximately 77-kD protein distinct from intracellular hsp58 and known members of the hsp70 stress protein family. While the exact nature and significance of this anti-hsp58-reactive protein remain to be determined, these data may help to clarify the roles of groEL-related stress proteins and gamma/delta cells that recognize groEL homologous in immunologic defense against infection and in autoimmune disease.
针对大肠杆菌应激蛋白groEL的人类同源物hsp58（P1）的兔抗体，在间接免疫荧光和补体依赖的微量细胞毒性实验中，与带有γ/δ受体的T细胞系组成性表达的一种细胞表面抗原发生特异性反应。这种抗hsp58反应性抗原在表达α/β受体的T细胞或缺乏T细胞受体的各种细胞上均未显示。已获得某些证据表明，γ/δ T细胞表面的靶抗原是一种约77-kD的蛋白质，与细胞内hsp58及hsp70应激蛋白家族的已知成员不同。虽然这种抗hsp58反应性蛋白的确切性质和意义尚待确定，但这些数据可能有助于阐明groEL相关应激蛋白以及识别groEL同源物的γ/δ细胞在抗感染免疫防御和自身免疫性疾病中的作用。
