妊娠期间及产后雌性大鼠膀胱和尿道中糖胺聚糖的代谢谱
Metabolic profile of glycosaminoglycans in bladder and urethra of female rats during and after pregnancy.
作者信息
Mitrano Presciliana, Nogueira Marie Doki, Feldner Paulo Cezar, Castro Rodrigo Aquino, Sartori Marair Gracio Ferreira, Nader Helena B, Girão Manoel João Batista Castello
机构信息
Section of Urogynecology and Pelvic Surgery, Department of Gynecology, Federal University of São Paulo, Rua dos Otonis, 601 Vila Clementino, 04025-001, São Paulo, SP, Brazil.
出版信息
Int Urogynecol J. 2010 Feb;21(2):241-6. doi: 10.1007/s00192-009-1001-z. Epub 2009 Oct 2.
INTRODUCTION AND HYPOTHESIS
The objective of this study is to evaluate pregnancy's action on glycosaminoglycans (GAGs) profile in bladder and urethra of female adult rats.
METHODS
Twenty adult female rats were divided into four groups: control, day 10 pregnancy, day 20 pregnancy, and day 5 after delivery. Sulfated GAGs content were determined by densitometry. Hyaluronic acid was assessed by fluorimetric enzyme-linked immunosorbent serologic-like assay. Radioactive GAGs were quantified by measuring radioactivity in electrophoresis gel. Statistical analysis was performed using Kruskal-Wallis and Mann-Whitney tests.
RESULTS
In all groups, dermatan sulfate was predominant followed by heparan sulfate and hyaluronic acid. Significant decrease in total GAGs in bladder was observed at the beginning of pregnancy, with progressive increase. Biosynthesis of such molecules showed a similar behavior. In urethra, changes in GAGs content were restricted to the end of pregnancy. Hyaluronic acid content showed a significant increase in bladder during puerperium.
CONCLUSIONS
We observed different composition in vesical and urethral tissues during pregnancy and in puerperium.
引言与假设
本研究的目的是评估妊娠对成年雌性大鼠膀胱和尿道中糖胺聚糖（GAGs）谱的影响。
方法
将20只成年雌性大鼠分为四组：对照组、妊娠第10天组、妊娠第20天组和产后第5天组。通过光密度法测定硫酸化GAGs含量。采用荧光酶联免疫吸附血清学样分析法评估透明质酸。通过测量电泳凝胶中的放射性对放射性GAGs进行定量。使用Kruskal-Wallis检验和Mann-Whitney检验进行统计分析。
结果
在所有组中，硫酸皮肤素占主导地位，其次是硫酸乙酰肝素和透明质酸。妊娠开始时膀胱中总GAGs显著减少，随后逐渐增加。这些分子的生物合成表现出类似的行为。在尿道中，GAGs含量的变化仅限于妊娠末期。产后膀胱中透明质酸含量显著增加。
结论
我们观察到妊娠期间和产后膀胱和尿道组织中的成分不同。
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