用于检测局限性粘附肠致病性大肠杆菌（EPEC）的EPEC粘附因子的寡核苷酸探针。
Oligonucleotide probe for detection of the enteropathogenic Escherichia coli (EPEC) adherence factor of localized adherent EPEC.
作者信息
Jerse A E, Martin W C, Galen J E, Kaper J B
机构信息
Department of Medicine, University of Maryland School of Medicine, Baltimore 21201.
出版信息
J Clin Microbiol. 1990 Dec;28(12):2842-4. doi: 10.1128/jcm.28.12.2842-2844.1990.
Abstract
The enteropathogenic Escherichia coli adherence factor (EAF) probe detects isolates of enteropathogenic E. coli that exhibit localized adherence to HEp-2 cells. A 21-base oligonucleotide probe was constructed on the basis of a sequence from within the 1-kb EAF probe and was shown to have greater sensitivity and specificity than the EAF fragment probe in detecting localized adherent E. coli.
摘要
肠致病性大肠杆菌黏附因子（EAF）探针可检测出对HEp-2细胞表现出局部黏附性的肠致病性大肠杆菌分离株。基于1 kb EAF探针内部的一段序列构建了一个21碱基的寡核苷酸探针，结果显示，在检测局部黏附性大肠杆菌时，该探针比EAF片段探针具有更高的灵敏度和特异性。
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