Stoll E, Billeter M A, Palmenberg A, Weissmann C
Cell. 1977 Sep;12(1):57-72. doi: 10.1016/0092-8674(77)90185-4.
We have determined the terminal heteropolymeric sequences of AMV RNA by the following procedures: first, RNA sequence determination on the 5' terminal and the poly(A)-linked 3' terminal T1 oligonucleotides, and second, analysis by the Maxam and Gilbert (1977) method of AMV strong stop DNA and of DNA complementary to the poly(A)-linked T1 oligonucleotide, synthesized with reverse transcriptase and (pdT)13 as primer. The structure deduced for the 5' terminal region is (5')7mGpppGmCCAUUCUACCUCUCACCACAUUGGUGUGCACCUGGGUUGAUGGCCGGACCGUCGAUUCCCUGACGACUACGAGCACCUGCAUGAAGCAGAAGGCUUCAU... Two distinct 3' terminal sequences were deduced: GCCAUUCUACCUCUCAAA...AOH and GCCAUUCUACCUCUCACCAAA...AOH. The two termini, differing by a C-C-A sequence, may reflect genetic heterogeneity of the AMV stock or, more probably, may be generated at or after RNA transcription. These results demonstrate a terminal redundancy of the hetero polymeric sequence of 16 and 19 nucleotides, respectively. The terminal redundancy allows for mechanisms which involve transfer of the DNA segment synthesized on the 5' terminal redundant sequence to the 3' terminal redundant sequence.
我们通过以下步骤确定了苜蓿花叶病毒（AMV）RNA的末端杂聚序列：首先，对5'末端和与多聚（A）相连的3'末端T1寡核苷酸进行RNA序列测定；其次，采用Maxam和Gilbert（1977年）的方法，对以逆转录酶和（pdT）13为引物合成的AMV强终止DNA以及与多聚（A）相连的T1寡核苷酸的互补DNA进行分析。推导得出的5'末端区域结构为（5'）7mGpppGmCCAUUCUACCUCUCACCACAUUGGUGUGCACCUGGGUUGAUGGCCGGACCGUCGAUUCCCUGACGACUACGAGCACCUGCAUGAAGCAGAAGGCUUCAU... 推导出两个不同的3'末端序列：GCCAUUCUACCUCUCAAA...AOH和GCCAUUCUACCUCUCACCAAA...AOH。这两个末端相差一个C-C-A序列，可能反映了AMV毒株的遗传异质性，或者更有可能是在RNA转录时或转录后产生的。这些结果分别证明了16个和19个核苷酸的杂聚序列存在末端冗余。末端冗余允许存在一些机制，这些机制涉及将在5'末端冗余序列上合成的DNA片段转移到3'末端冗余序列上。
