Ferris Nigel P, Nordengrahn Ann, Hutchings Geoffrey H, Reid Scott M, King Donald P, Ebert Katja, Paton David J, Kristersson Therese, Brocchi Emiliana, Grazioli Santina, Merza Malik
Institute for Animal Health, Pirbright Laboratory, Ash Road, Pirbright, Woking, Surrey GU24 0NF, UK.
J Virol Methods. 2009 Jan;155(1):10-7. doi: 10.1016/j.jviromet.2008.09.009. Epub 2008 Nov 5.
A lateral flow device (LFD) for the detection of all seven serotypes of foot-and-mouth disease virus (FMDV) was developed using a monoclonal antibody (Mab 1F10) shown to be pan-reactive to FMDV strains of each serotype by ELISA. The performance of the LFD was evaluated in the laboratory on suspensions of vesicular epithelia (304 positive and 1003 negative samples) from suspected cases of vesicular disease collected from 86 countries between 1965 and 2008 and negative samples collected from healthy animals. The diagnostic sensitivity of the LFD for FMDV was similar at 84% compared to 85% obtained by the reference method of antigen ELISA, and the diagnostic specificity of the LFD was approximately 99% compared to 99.9% for the ELISA. The device recognized FMDV strains of wide diversity of all seven serotypes but weaker reactions were often evident with those of type SAT 2, several viruses of which were not detected. Reactions with the viruses of swine vesicular disease and vesicular stomatitis that produce clinically indistinguishable syndromes in pigs and cattle, did not occur. The test procedure was simple and rapid, and typically provided a result within 1-10min of sample addition. Simple homogenizers that could be used in field conditions for preparing epithelial suspensions were demonstrated to be effective for LFD application. These data illustrate the potential for the LFD to be used next to the animal in the pen-side diagnosis of FMD and for providing rapid and objective support to veterinarians in their clinical judgment of the disease.
利用一种单克隆抗体（Mab 1F10）开发了一种用于检测口蹄疫病毒（FMDV）所有七种血清型的侧向流动装置（LFD），该抗体通过酶联免疫吸附测定（ELISA）显示对每种血清型的FMDV毒株具有泛反应性。在实验室中，对1965年至2008年间从86个国家收集的疑似水疱病病例的水疱上皮悬液（304份阳性和1003份阴性样本）以及从健康动物收集的阴性样本，评估了LFD的性能。LFD对FMDV的诊断敏感性为84%，与抗原ELISA参考方法获得的85%相似，LFD的诊断特异性约为99%，而ELISA为99.9%。该装置可识别所有七种血清型的多种FMDV毒株，但对SAT 2型毒株的反应通常较弱，其中几种病毒未被检测到。与猪水疱病病毒和水疱性口炎病毒没有交叉反应，这两种病毒在猪和牛身上产生临床上难以区分的综合征。检测过程简单快速，通常在加入样本后1 - 10分钟内得出结果。已证明可在现场条件下用于制备上皮悬液的简单匀浆器对LFD应用有效。这些数据表明LFD有潜力用于在畜栏旁对动物进行口蹄疫的诊断，并为兽医在疾病临床判断中提供快速客观的支持。
