College of Animal Science and Veterinary Medicine, Henan Agricultural University, No. 95 Wenhua Road, Zhengzhou 450002, China.
Appl Environ Microbiol. 2009 Dec;75(24):7692-9. doi: 10.1128/AEM.01386-09. Epub 2009 Oct 9.
To understand the prevalence of Cryptosporidium infection in rodents in China and to assess the potential role of rodents as a source for human cryptosporidiosis, 723 specimens from 18 rodent species were collected from four provinces of China and examined between August 2007 and December 2008 by microscopy after using Sheather's sugar flotation and modified acid-fast staining. Cryptosporidium oocysts were detected in 83 specimens, with an overall prevalence of 11.5%. Phodopus sungorus, Phodopus campbelli, and Rattus tanezumi were new reported hosts of Cryptosporidium. The genotypes and subtypes of Cryptosporidium strains in microscopy-positive specimens were further identified by PCR and sequence analysis of the small subunit rRNA and the 60-kDa glycoprotein (gp60) genes. In addition to Cryptosporidium parvum, C. muris, C. andersoni, C. wrairi, ferret genotype, and mouse genotype I, four new Cryptosporidium genotypes were identified, including the hamster genotype, chipmunk genotype III, and rat genotypes II and III. Mixed Cryptosporidium species/genotypes were found in 10.8% of Cryptosporidium-positive specimens. Sequence analysis of the gp60 gene showed that C. parvum strains in pet Siberian chipmunks and hamsters were all of the subtype IIdA15G1, which was found previously in a human isolate in The Netherlands and lambs in Spain. The gp60 sequences of C. wrairi and the Cryptosporidium ferret genotype and mouse genotype I were also obtained. These findings suggest that pet rodents may be potential reservoirs of zoonotic Cryptosporidium species and subtypes.
为了解中国啮齿动物中隐孢子虫感染的流行情况，并评估啮齿动物作为人类隐孢子虫病传染源的潜在作用，我们于 2007 年 8 月至 2008 年 12 月期间，从中国四个省份的 18 种啮齿动物中采集了 723 个标本，使用 Sheather 的蔗糖漂浮法和改良抗酸染色法进行显微镜检查。在 83 个标本中检测到隐孢子虫卵囊，总流行率为 11.5%。黑线毛足鼠、甘肃仓鼠和黑线姬鼠是隐孢子虫的新宿主。通过 PCR 和小亚单位 rRNA 及 60-kDa 糖蛋白（gp60）基因序列分析，对显微镜阳性标本中的隐孢子虫株的基因型和亚型进行了进一步鉴定。除了微小隐孢子虫、鼠隐孢子虫、鼠隐孢子虫、等孢球虫、雪貂基因型和鼠基因型 I 以外，还鉴定出了四种新的隐孢子虫基因型，包括仓鼠基因型、花栗鼠基因型 III 和大鼠基因型 II 和 III。在 10.8%的隐孢子虫阳性标本中发现了混合隐孢子虫种/基因型。gp60 基因序列分析显示，宠物西伯利亚花栗鼠和仓鼠中的微小隐孢子虫株均为亚型 IIdA15G1，该亚型之前在荷兰的人类分离株和西班牙的绵羊中发现过。还获得了等孢球虫和隐孢子虫雪貂基因型及鼠基因型 I 的 gp60 序列。这些发现表明，宠物啮齿动物可能是潜在的人畜共患隐孢子虫种和亚型的储存宿主。
