激活的人类基因上的新生转录波。
A wave of nascent transcription on activated human genes.
作者信息
Wada Youichiro, Ohta Yoshihiro, Xu Meng, Tsutsumi Shuichi, Minami Takashi, Inoue Kenji, Komura Daisuke, Kitakami Jun'ichi, Oshida Nobuhiko, Papantonis Argyris, Izumi Akashi, Kobayashi Mika, Meguro Hiroko, Kanki Yasuharu, Mimura Imari, Yamamoto Kazuki, Mataki Chikage, Hamakubo Takao, Shirahige Katsuhiko, Aburatani Hiroyuki, Kimura Hiroshi, Kodama Tatsuhiko, Cook Peter R, Ihara Sigeo
机构信息
Laboratory for Systems Biology and Medicine, Research Center for Advanced Science and Technology, The University of Tokyo, Tokyo, Japan.
出版信息
Proc Natl Acad Sci U S A. 2009 Oct 27;106(43):18357-61. doi: 10.1073/pnas.0902573106. Epub 2009 Oct 13.
Genome-wide studies reveal that transcription by RNA polymerase II (Pol II) is dynamically regulated. To obtain a comprehensive view of a single transcription cycle, we switched on transcription of five long human genes (>100 kbp) with tumor necrosis factor-alpha (TNFalpha) and monitored (using microarrays, RNA fluorescence in situ hybridization, and chromatin immunoprecipitation) the appearance of nascent RNA, changes in binding of Pol II and two insulators (the cohesin subunit RAD21 and the CCCTC-binding factor CTCF), and modifications of histone H3. Activation triggers a wave of transcription that sweeps along the genes at approximately 3.1 kbp/min; splicing occurs cotranscriptionally, a major checkpoint acts several kilobases downstream of the transcription start site to regulate polymerase transit, and Pol II tends to stall at cohesin/CTCF binding sites.
全基因组研究表明，RNA聚合酶II（Pol II）介导的转录是动态调控的。为全面了解单个转录周期，我们用肿瘤坏死因子-α（TNFα）开启了五个长人类基因（>100 kbp）的转录，并（使用微阵列、RNA荧光原位杂交和染色质免疫沉淀）监测新生RNA的出现、Pol II和两种绝缘子（黏连蛋白亚基RAD21和CCCTC结合因子CTCF）结合的变化以及组蛋白H3的修饰。激活引发一波转录，以约3.1 kbp/分钟的速度沿基因推进；剪接在转录过程中发生，一个主要检查点在转录起始位点下游数千碱基处起作用以调节聚合酶的行进，并且Pol II倾向于在黏连蛋白/CTCF结合位点处停滞。
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