School of Dental Hygiene, Old Dominion University, Norfolk, VA, USA.
Int J Dent Hyg. 2009 Nov;7(4):241-5. doi: 10.1111/j.1601-5037.2008.00352.x.
This project was conducted to determine the effectiveness of chlorhexidine-coated toothbrush filaments in reducing quantities of bacteria.
An Institutional Review Board (IRB)-approved, two-group, double-blind, randomized, post-test only study was conducted. Sixty-four individuals utilized control and experimental toothbrushes, for 30 days. At the end of the study toothbrushes were returned and transported to the laboratory for analysis. Microorganisms were detached from the filaments by sonification and vortexing then plated on Mitis Salivarius (MS) (selective) and trypticase soy agar (TSA) 5% Sheep Blood (non-selective) media. Inoculated plates were incubated aerobically for 24 h at 37 degrees C. After incubation, bacterial colony-forming units (CFU) were determined. Data were analysed using Wilcoxon and Kruskal-Wallis tests.
Fifty-nine toothbrushes were returned for analysis; experimental (n = 31) and control (n = 28). Data from TSA media revealed a mean CFU for the control group of 5.41 x 10(5) compared with 6.28 x 10(5) for the experimental group. Data from MS agar resulted in a mean CFU for the control group of 4.32 x 10(5) compared with 4.20 x 10(5) for the experimental group.
Results revealed no statistically significant difference in the quantity of bacteria surviving on toothbrush filaments between control and experimental groups, on both selective and non-selective media, after 30 days.
本项目旨在确定氯己定涂层牙刷丝在减少细菌数量方面的有效性。
进行了一项机构审查委员会（IRB）批准的、两组、双盲、随机、仅后测试研究。64 名个体使用对照和实验组牙刷，为期 30 天。研究结束时，牙刷被退回并运送到实验室进行分析。通过超声处理和涡旋将微生物从丝上分离出来，然后接种到米氏链球菌（MS）（选择性）和胰蛋白酶大豆琼脂（TSA）5%绵羊血（非选择性）培养基上。接种的平板在 37°C 下需有氧孵育 24 小时。孵育后，确定细菌菌落形成单位（CFU）。使用 Wilcoxon 和 Kruskal-Wallis 检验分析数据。
有 59 支牙刷被送回进行分析；实验组（n=31）和对照组（n=28）。来自 TSA 培养基的数据显示，对照组的平均 CFU 为 5.41 x 10(5)，而实验组的平均 CFU 为 6.28 x 10(5)。来自 MS 琼脂的数据得出对照组的平均 CFU 为 4.32 x 10(5)，而实验组的平均 CFU 为 4.20 x 10(5)。
结果显示，在 30 天后，两种选择性和非选择性培养基上，对照组和实验组牙刷丝上存活的细菌数量没有统计学上的显著差异。
