Beltman Joost B, Marée Athanasius F M, de Boer Rob J
Theoretical Biology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
Nat Rev Immunol. 2009 Nov;9(11):789-98. doi: 10.1038/nri2638. Epub 2009 Oct 16.
The visualization of the dynamic behaviour of and interactions between immune cells using time-lapse video microscopy has an important role in modern immunology. To draw robust conclusions, quantification of such cell migration is required. However, imaging experiments are associated with various artefacts that can affect the estimated positions of the immune cells under analysis, which form the basis of any subsequent analysis. Here, we describe potential artefacts that could affect the interpretation of data sets on immune cell migration. We propose how these errors can be recognized and corrected, and suggest ways to prevent the data analysis itself leading to biased results.
使用延时视频显微镜观察免疫细胞的动态行为及其之间的相互作用在现代免疫学中具有重要作用。为了得出可靠的结论，需要对这种细胞迁移进行量化。然而，成像实验会出现各种伪像，这些伪像会影响所分析免疫细胞的估计位置，而这些位置是后续任何分析的基础。在这里，我们描述了可能影响免疫细胞迁移数据集解释的潜在伪像。我们提出了如何识别和纠正这些误差，并建议了防止数据分析本身导致有偏差结果的方法。
