Molecular Endocrinology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, Uttarakhand, India.
J Ethnopharmacol. 2010 Feb 3;127(2):457-62. doi: 10.1016/j.jep.2009.10.013. Epub 2009 Oct 25.
Capparis decidua (CD) is a xerophytic shrub, found widely in the western parts of India, Pakistan and some of the Asian countries. The dried fruits are used as an ingredient in anti-diabetic compositions.
The present study was carried out to test the effect of alkaloid rich (AR) fraction from this plant in the management of diabetes.
Streptozotocin-induced diabetic mice were treated with the AR fraction for 28 days. On completion of the treatment, a range of parameters were tested including oral glucose tolerance test (OGTT), blood lipid profile, expression patterns of various glucose homeostatic enzyme genes and their activities.
Treatment of diabetic mice with AR fraction for 28 days significantly inhibited the acute elevation of blood glucose level during OGTT and also reduced total cholesterol (TC) and triglyceride (TG) content (p<0.05). Activity of glucose-6-phosphatase (G6Pase) was attenuated by 44%, also liver and muscle glycogen content showed significant improvement (p<0.05). The expression of different target genes like G6Pase, phosphoenolpyruvate carboxykinase (PEPCK), aldose reductase and tumor necrosis factor-alpha (TNF-alpha) showed significant reduction whereas glucose transporter-4 (Glut-4), peroxisome proliferator activated receptor-gamma (PPAR-gamma) and glucokinase (GK) improved remarkably.
AR fraction showed promising results in terms of anti-diabetic activities establishing its candidacy for further purification and characterization of the individual alkaloids, in order to understand their mechanism of action.
角茴香（CD）是一种旱生灌木，广泛分布于印度西部、巴基斯坦和一些亚洲国家。其干果实被用作抗糖尿病成分。
本研究旨在测试该植物富含生物碱（AR）部分在糖尿病治疗中的作用。
用 AR 部分处理链脲佐菌素诱导的糖尿病小鼠 28 天。治疗结束时，测试了一系列参数，包括口服葡萄糖耐量试验（OGTT）、血脂谱、各种葡萄糖稳态酶基因的表达模式及其活性。
用 AR 部分治疗糖尿病小鼠 28 天可显著抑制 OGTT 期间血糖水平的急性升高，并降低总胆固醇（TC）和甘油三酯（TG）含量（p<0.05）。葡萄糖-6-磷酸酶（G6Pase）活性降低 44%，肝和肌肉糖原含量也显著改善（p<0.05）。不同靶基因如 G6Pase、磷酸烯醇丙酮酸羧激酶（PEPCK）、醛糖还原酶和肿瘤坏死因子-α（TNF-α）的表达显著降低，而葡萄糖转运蛋白-4（Glut-4）、过氧化物酶体增殖物激活受体-γ（PPAR-γ）和葡萄糖激酶（GK）显著改善。
AR 部分在抗糖尿病活性方面表现出有希望的结果，为进一步纯化和表征个别生物碱奠定了基础，以了解其作用机制。
