Ito Y, Montagnier L
Infect Immun. 1977 Oct;18(1):23-7. doi: 10.1128/iai.18.1.23-27.1977.
Twelve cloned viruses were randomly isolated from original (uncloned) vesicular stomatitis virus (VSV), and their sensitivities to mouse and human interferons were examined. When the interferon sensitivities of these various VSVs were compared by the plaque reduction method in L cells, virus 3 was found to be sevenfold more sensitive than virus 11, and the interferon sensitivity of the original (uncloned) virus was intermediate. The present study shows that uncloned VSV Indiana strain is a mixture of viruses that have different sensitivities to interferon. The slope of the dose-response curve of original (uncloned) virus to mouse interferon was less steep than those of cloned viruses. Virus 11, which was the least sensitive to mouse interferon, was relatively sensitive to human interferon. There was no correlation between the sensitivities of virus clones to mouse interferon and their sensitivities to human interferon. When the interferon sensitivities were tested by various assay methods (plaque reduction, yield reduction, and cytopathic effect inhibition), the ranking of the interferon sensitivities of different viruses was not changed. These results indicate that the relative sensitivity of a virus to interferon is determined by the host cells in which the tests are performed, but not by assay method used.
从原始（未克隆）水疱性口炎病毒（VSV）中随机分离出12种克隆病毒，并检测了它们对小鼠和人干扰素的敏感性。当通过空斑减少法在L细胞中比较这些不同VSV的干扰素敏感性时，发现病毒3比病毒11敏感7倍，原始（未克隆）病毒的干扰素敏感性处于中间水平。本研究表明，未克隆的VSV印第安纳株是对干扰素敏感性不同的病毒混合物。原始（未克隆）病毒对小鼠干扰素的剂量反应曲线斜率比克隆病毒的斜率平缓。对小鼠干扰素最不敏感的病毒11对人干扰素相对敏感。病毒克隆对小鼠干扰素的敏感性与其对人干扰素的敏感性之间没有相关性。当通过各种检测方法（空斑减少、产量减少和细胞病变效应抑制）检测干扰素敏感性时，不同病毒的干扰素敏感性排名没有改变。这些结果表明，病毒对干扰素的相对敏感性由进行检测的宿主细胞决定，而非所用的检测方法。
