Franzblau S G, Harris E B
Laboratory Research Branch, Gillis W. Long Hansen's Disease Center, Carville, Louisiana 70721.
J Clin Microbiol. 1988 Jun;26(6):1124-9. doi: 10.1128/jcm.26.6.1124-1129.1988.
The metabolic response of freshly harvested, nude-mouse-derived Mycobacterium leprae to biophysical parameters was studied to facilitate an understanding of axenic culture requirements. Quantitation of intracellular ATP and the rate of [U-14C]palmitic acid incorporation into phenolic glycolipid I (PGL-I) were used as metabolic indicators after axenic incubation in modified Dubos medium under various biophysical conditions. PGL-I synthesis was optimal at 33 degrees C, whereas ATP was optimally maintained at less than or equal to 33 degrees C. Both metabolic indices showed sharp reductions at 37 degrees C. After 5 days of incubation, PGL-I synthesis and ATP maintenance showed pH optima of 5.1 to 5.6, with the higher value appearing optimal for ATP maintenance after extended incubation. Metabolic activity was negatively affected by strong reducing agents, and ATP maintenance was optimal when the gaseous environment was maintained at 2.5 to 10% oxygen. The results may partially explain the failure to cultivate the leprosy bacillus in vitro.
研究了刚收获的、源自裸鼠的麻风分枝杆菌对生物物理参数的代谢反应，以促进对无菌培养条件的理解。在各种生物物理条件下，于改良的杜博斯培养基中进行无菌培养后，将细胞内ATP的定量以及[U-14C]棕榈酸掺入酚糖脂I（PGL-I）的速率用作代谢指标。PGL-I合成在33℃时最佳，而ATP在小于或等于33℃时维持最佳水平。两种代谢指标在37℃时均急剧下降。培养5天后，PGL-I合成和ATP维持的最适pH为5.1至5.6，延长培养后较高的值似乎对ATP维持最为适宜。代谢活性受到强还原剂的负面影响，当气体环境维持在2.5%至10%的氧气时，ATP维持最佳。这些结果可能部分解释了体外培养麻风杆菌失败的原因。
