冠状病毒HCoV-NL63在细胞培养中的形态发生：透射电子显微镜研究
Morphogenesis of Coronavirus HCoV-NL63 in Cell Culture: A Transmission Electron Microscopic Study.
作者信息
Orenstein Jan M, Banach Bridget, Baker Susan C
机构信息
Department of Pathology, George Washington University School of Medicine, 2300 Eye Street NW, Washington, DC, USA.
出版信息
Open Infect Dis J. 2008 Jan 1;2:52-58. doi: 10.2174/1874279300802010052.
Abstract
NL63 (HCoV-NL63) is a recently discovered human coronavirus that causes respiratory disease in infants and young children. NL63 productively infects LLCMK2 cells and ciliated epithelial cells of human airway cell cultures. Transmission electron microscopic (TEM) studies of NL63 infected LLCMK2 cells revealed that virions are spherical, spiked, and range from 75 to 115 nm in diameter. Virus replication predominantly occurs on the rough endoplasmic reticulum (RER), both perinuclear and cytoplasmic, and the Golgi. Plasma membrane budding was occasionally observed. As virus production increased, aberrant viral forms appeared with greater frequency. Unusual inclusions were present in infected cells including tubular and laminated structures. Pleomorphic double membrane-bound vesicles (DMV), measuring roughly 140 to 210 nm in diameter, were observed. The virus was released via exocytosis and cell lysis. In summary, we report the key morphologic characteristics of NL63 infection observed by TEM analysis.
摘要
NL63（人冠状病毒NL63）是一种最近发现的可导致婴幼儿呼吸道疾病的人类冠状病毒。NL63能有效感染LLCMK2细胞和人气道细胞培养物中的纤毛上皮细胞。对感染NL63的LLCMK2细胞进行的透射电子显微镜（TEM）研究显示，病毒粒子呈球形，有刺突，直径在75至115纳米之间。病毒复制主要发生在核周和细胞质中的糙面内质网（RER）以及高尔基体上。偶尔可观察到质膜出芽现象。随着病毒产量的增加，异常病毒形式出现的频率更高。受感染细胞中存在异常包涵体，包括管状和层状结构。观察到直径约为140至210纳米的多形性双膜结合囊泡（DMV）。病毒通过胞吐作用和细胞裂解释放。总之，我们报告了通过TEM分析观察到的NL63感染的关键形态学特征。
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