Department of Pesticide Chemistry and Toxicology, Alexandria University, Alexandria 55555, Egypt.
Reprod Toxicol. 2010 Jan;29(1):80-5. doi: 10.1016/j.reprotox.2009.10.003. Epub 2009 Oct 20.
The reproductive toxicity of the insecticide chloropyrifos was studied in male mice. Adult male mice were treated by gavage with chloropyrifos at doses of 0, 5, 15, and 25mg/kg-d for 4 weeks before mating with untreated females. Signs of cholinergic effects were observed in the 15 and 25mg/kg-d treated groups. Brain and skeletal muscle acetylcholinesterase activity was inhibited in the same groups. Chloropyrifos treatment was associated with a decreased number of live fetuses, and an increased number of dead fetuses at 25mg/kg-d. The number of early resorption was decreased in both 15 and 25mg/kg-d treated groups. The percent morphologically normal spermatozoa were affected in 15 and 25mg/kg-d dose groups; however, sperm motility and count were decreased in the same treated groups compared to the control. Histologic examination of brain revealed histological abnormalities in the middle and high groups. Dose related histologic changes, including degeneration of muscle fibers, were observed in the muscles of male mice treated with all the doses of chloropyrifos. The current study demonstrated adverse effects of male chloropyrifos exposure on pregnancy outcome with effects on sperm parameters at 15 and 25mg/kg-d.
本研究旨在探讨杀虫剂氯吡硫磷对雄性小鼠的生殖毒性。成年雄性小鼠经灌胃给予氯吡硫磷 0、5、15 和 25mg/kg-d，连续处理 4 周后与未经处理的雌性小鼠交配。在 15 和 25mg/kg-d 处理组中观察到胆碱能效应的迹象。同一组的大脑和骨骼肌乙酰胆碱酯酶活性受到抑制。氯吡硫磷处理与活胎数量减少和 25mg/kg-d 处理组死胎数量增加有关。在 15 和 25mg/kg-d 处理组中，早期吸收的数量减少。15 和 25mg/kg-d 剂量组的形态正常精子数量受到影响；然而，与对照组相比，同一处理组的精子活力和计数下降。大脑的组织学检查显示中高剂量组存在组织学异常。在接受氯吡硫磷所有剂量处理的雄性小鼠的肌肉中观察到与剂量相关的组织学变化，包括肌纤维变性。本研究表明，雄性接触氯吡硫磷会对妊娠结局产生不利影响，在 15 和 25mg/kg-d 剂量下会影响精子参数。
