Center for Emerging Infectious Diseases, Department of Epidemiology, University of Iowa College of Public Health, Iowa City, IA 52241, USA.
J Clin Virol. 2009 Dec;46(4):331-6. doi: 10.1016/j.jcv.2009.09.023. Epub 2009 Oct 23.
Adenovirus type 3 (HAdV3) is one of the most prevalent serotypes detected globally. Variants of HAdV3 have been associated with outbreaks of severe disease.
To better understand genetic diversity of circulating HAdV3s and examine risk factors for severe disease.
Restriction enzyme analysis for genomic characterization of clinical HAdV3 isolates detected by 15 collaborative US laboratories during the period July 2004 to May 2007. Multivariate modeling was employed for statistical analyses.
The most common HAdV3 types of 516 isolates studied were HAdV3a2 (36.9%), HAdV3a50 (27.1%), HAdV3a51 (18.0%), and HAdV3a17 (4.6%). Non-HAdV3a genome types were rare (1.2%). HAdV3a50 and HAdV3a51 are newly described variants which became more prevalent in 2006 and 2007 and have been associated with at least one epidemic. Their uniqueness was determined by specific banding profiles generated by digests with endonucleases BclI, BglII, and HindIII. Multivariable risk factor modeling demonstrated that children under 2 years of age (OR=2.7; 95%CI 1.6-4.6), persons with chronic disease (OR=5.1; 95%CI 2.6-9.8), persons infected with HAdV3a2 (OR=3.0; 95%CI 1.5-6.0), with HAdV3a50 (OR=2.5; 95%CI 1.2-5.2), or with multiple or rare strains (OR=2.8; 95%CI 1.3-6.5) were at increased risk of severe HAdV3 clinical disease.
In the study period considerable genetic diversity was found among US clinical HAdV3 strains. Novel variants emerged and became prevalent. One such emergent strain may be associated with more severe clinical disease.
腺病毒 3 型（HAdV3）是全球最常见的血清型之一。HAdV3 的变异株与严重疾病的爆发有关。
更好地了解循环 HAdV3 的遗传多样性，并研究严重疾病的危险因素。
对 2004 年 7 月至 2007 年 5 月期间，美国 15 个合作实验室检测到的临床 HAdV3 分离株进行基因组特征的限制性内切酶分析。采用多变量建模进行统计分析。
在所研究的 516 株中，最常见的 HAdV3 类型为 HAdV3a2（36.9%）、HAdV3a50（27.1%）、HAdV3a51（18.0%）和 HAdV3a17（4.6%）。非 HAdV3a 基因组类型很少见（1.2%）。HAdV3a50 和 HAdV3a51 是新描述的变异株，它们在 2006 年和 2007 年变得更为普遍，并与至少一次流行有关。它们的独特性是由内切酶 BclI、BglII 和 HindIII 消化产生的特定带型决定的。多变量危险因素模型表明，2 岁以下儿童（OR=2.7；95%CI 1.6-4.6）、慢性病患者（OR=5.1；95%CI 2.6-9.8）、感染 HAdV3a2 的患者（OR=3.0；95%CI 1.5-6.0）、感染 HAdV3a50 的患者（OR=2.5；95%CI 1.2-5.2）或感染多种或罕见株的患者（OR=2.8；95%CI 1.3-6.5）发生严重 HAdV3 临床疾病的风险增加。
在研究期间，发现美国临床 HAdV3 株存在相当大的遗传多样性。新型变异株出现并流行。其中一种新兴株可能与更严重的临床疾病有关。
