Jones Morris Saffold, Harrach Balázs, Ganac Robert D, Gozum Mary M A, Dela Cruz Wilfred P, Riedel Brian, Pan Chao, Delwart Eric L, Schnurr David P
Clinical Investigation Facility, David Grant USAF Medical Center, 101 Bodin Circle, Travis AFB, CA 94535, USA.
J Virol. 2007 Jun;81(11):5978-84. doi: 10.1128/JVI.02650-06. Epub 2007 Mar 14.
An unidentified agent was cultured in primary monkey cells at the Los Angeles County Public Health Department from each of five stool specimens submitted from an outbreak of gastroenteritis. Electron microscopy and an adenovirus-specific monoclonal antibody confirmed this agent to be an adenovirus. Since viral titers were too low, complete serotyping was not possible. Using the DNase-sequence-independent viral nucleic acid amplification method, we identified several nucleotide sequences with a high homology to human adenovirus 41 (HAdV-41) and simian adenovirus 1 (SAdV-1). However, using anti-SAdV-1 sera, it was determined that this virus was serologically different than SAdV-1. Genomic sequencing and phylogenetic analysis confirmed that this new adenovirus was so divergent from the known human adenoviruses that it was not only a new type but also represented a new species (human adenovirus G). In a retrospective clinical study, this new virus was detected by PCR in one additional patient from a separate gastroenteritis outbreak. This study suggests that HAdV-52 may be one of many agents causing gastroenteritis of unknown etiology.
从一次胃肠炎暴发中提交的五份粪便标本中，洛杉矶县公共卫生部门在原代猴细胞中培养出一种未知病原体。电子显微镜检查和腺病毒特异性单克隆抗体证实该病原体为腺病毒。由于病毒滴度太低，无法进行完整的血清分型。使用不依赖核酸酶序列的病毒核酸扩增方法，我们鉴定出了几个与人类腺病毒41（HAdV-41）和猴腺病毒1（SAdV-1）具有高度同源性的核苷酸序列。然而，使用抗SAdV-1血清检测发现，这种病毒在血清学上与SAdV-1不同。基因组测序和系统发育分析证实，这种新的腺病毒与已知的人类腺病毒差异极大，它不仅是一种新型病毒，还代表了一个新的种（人类腺病毒G）。在一项回顾性临床研究中，通过聚合酶链反应（PCR）在另一起单独的胃肠炎暴发中的一名患者体内检测到了这种新病毒。这项研究表明，HAdV-52可能是许多导致病因不明的胃肠炎的病原体之一。
