Tong Ling, He Wei, Zhang Yanshu, Zheng Wei, Cheng Ji-Xin
Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA.
Langmuir. 2009 Nov 3;25(21):12454-9. doi: 10.1021/la902992w.
Characterization of systemic performance of gold nanostructures is critical to the advancement of biomedical applications of these nanomaterials as imaging or therapeutic agents. The accuracy of current in vitro methods, however, is limited by interanimal variability. We present a novel method capable of monitoring the pharmacokinetics of PEGylated gold nanorods (GNRs) in the same animal by using intravital two-photon luminescence (TPL) imaging of GNRs flowing through a surface blood vessel. The TPL imaging with high speed and submicrometer resolution allowed for studying the clearance of GNRs as a function of surface coating. PEGylated-GNRs (PEG-NRs) were found to exhibit a biphasic clearance mode, with a significantly prolonged blood residence time for branched poly(ethylene glycol) (PEG) as compared to the linear PEG. With spectral detection to distinguish GNR TPL from tissue autofluorescence, we also mapped the cellular distribution of GNRs in the explanted organs, and found most GNRs resided in the macrophages in liver and spleen.
对金纳米结构的全身性能进行表征对于将这些纳米材料作为成像或治疗剂推进生物医学应用至关重要。然而，当前体外方法的准确性受到动物个体间差异的限制。我们提出了一种新方法，通过对流经表面血管的金纳米棒（GNR）进行活体双光子发光（TPL）成像，能够在同一只动物体内监测聚乙二醇化金纳米棒（PEG-GNR）的药代动力学。具有高速和亚微米分辨率的TPL成像使得研究GNR的清除率与表面涂层的关系成为可能。发现聚乙二醇化金纳米棒（PEG-NR）呈现双相清除模式，与线性聚乙二醇相比，支化聚（乙二醇）（PEG）的血液停留时间显著延长。通过光谱检测以区分GNR的TPL和组织自发荧光，我们还绘制了GNR在离体器官中的细胞分布，发现大多数GNR存在于肝脏和脾脏的巨噬细胞中。
