Department of Drug Sciences, School of Pharmacy, G d'Annunzio University, Chieti, Italy.
J Med Food. 2009 Oct;12(5):1166-9. doi: 10.1089/jmf.2008.0176.
Oxidative stress plays a key role in the pathogenesis of age-related neurodegeneration, and the nonenzymatic production of 8-iso-prostaglandin F(2alpha) (8-iso-PGF(2alpha)) may represent a reliable index of cellular oxidative damage. Garlic (Allium sativum) has been associated with peripheral antioxidant activities and therefore might prevent or reverse 8-iso-PGF(2alpha) production, but scant data are available on its possible neuroprotective effects. Therefore, we have studied the possible antioxidant effects of a garlic extract in rat brain synaptosomes obtained from young (3-month-old) and aged (14-month-old) male Wistar rats that were perfused, in vitro, with graded concentrations of a garlic extract (10-500 microg/mL). Release in the effluent was evaluated, both in the basal state and after hydrogen peroxide-induced oxidative stress. In young rats, we observed a concentration-dependent inhibitory effect of the garlic extract on brain 8-iso-PGF(2alpha) production, both basally and after hydrogen peroxide-induced oxidative stimulus. In aged rats, 8-iso-PGF(2alpha) production was not affected by the garlic extract in the basal state, whereas, after hydrogen peroxide-induced oxidative stimulus, an antioxidant effect of the garlic extract appeared only at the higher concentration tested. In conclusion, garlic supplementation could be effective in preventing brain oxidative damage in young animals, whereas the aging brain seems to be resistant to the antioxidant effects of garlic, in vitro.
氧化应激在与年龄相关的神经退行性变的发病机制中起着关键作用，非酶产生的 8-异前列腺素 F2alpha（8-iso-PGF2alpha）可能是细胞氧化损伤的一个可靠指标。大蒜（Allium sativum）与外周抗氧化活性有关，因此可能预防或逆转 8-iso-PGF2alpha 的产生，但关于其可能的神经保护作用的数据很少。因此，我们研究了大蒜提取物在年轻（3 个月大）和年老（14 个月大）雄性 Wistar 大鼠脑突触体中的可能抗氧化作用，这些大鼠在体外用不同浓度的大蒜提取物（10-500μg/mL）灌流。在基础状态和过氧化氢诱导的氧化应激后，评估了流出物中的释放。在年轻大鼠中，我们观察到大蒜提取物对大脑 8-iso-PGF2alpha 产生的浓度依赖性抑制作用，无论是在基础状态还是在过氧化氢诱导的氧化刺激后。在年老大鼠中，大蒜提取物在基础状态下不影响 8-iso-PGF2alpha 的产生，但在过氧化氢诱导的氧化应激后，大蒜提取物的抗氧化作用仅在测试的较高浓度下出现。总之，大蒜补充剂可能有效预防年轻动物的大脑氧化损伤，而衰老的大脑似乎对大蒜的抗氧化作用具有抗性，体外。
