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通过体内足迹法检测到PUT3转录激活蛋白的脯氨酸非依赖性结合。

Proline-independent binding of PUT3 transcriptional activator protein detected by footprinting in vivo.

作者信息

Axelrod J D, Majors J, Brandriss M C

机构信息

Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri 63110.

出版信息

Mol Cell Biol. 1991 Jan;11(1):564-7. doi: 10.1128/mcb.11.1.564-567.1991.

DOI:10.1128/mcb.11.1.564-567.1991
PMID:1986247
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC359669/
Abstract

The PUT3 gene product is a transcriptional activator required for expression of the enzymes of the proline utilization pathway. Using two methods of footprinting in vivo, we have determined that PUT3 protein is poised at the promoters of the genes encoding these enzymes and that proline-mediated induction modulates the activity of constitutively bound PUT3.

摘要

PUT3基因产物是脯氨酸利用途径中酶表达所需的转录激活因子。通过两种体内足迹法，我们确定PUT3蛋白定位于编码这些酶的基因启动子处，并且脯氨酸介导的诱导调节组成型结合的PUT3的活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a22/359669/4b97f22ce52c/molcellb00136-0578-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a22/359669/7ff4d853a29b/molcellb00136-0577-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a22/359669/4b97f22ce52c/molcellb00136-0578-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a22/359669/7ff4d853a29b/molcellb00136-0577-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a22/359669/4b97f22ce52c/molcellb00136-0578-a.jpg

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Use of light for footprinting DNA in vivo.利用光在体内对DNA进行足迹分析。
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