Sun Li-Yi, Hsieh Dean-Kuo, Lin Po-Cheng, Chiu Hsien-Tai, Chiou Tzyy-Wen
Department of Biological Science and Technology, National Chiao Tung University, No. 75 Po-Ai Street, Hsinchu, Taiwan, ROC.
Bioelectromagnetics. 2010 Apr;31(3):209-19. doi: 10.1002/bem.20550.
Osteogenesis is a complex series of events involving the differentiation of mesenchymal stem cells to generate new bone. In this study, we examined the effect of pulsed electromagnetic fields (PEMFs) on cell proliferation, alkaline phosphatase (ALP) activity, mineralization of the extracellular matrix, and gene expression in bone marrow mesenchymal stem cells (BMMSCs) during osteogenic differentiation. Exposure of BMMSCs to PEMFs increased cell proliferation by 29.6% compared to untreated cells at day 1 of differentiation. Semi-quantitative RT-PCR indicated that PEMFs significantly altered temporal expression of osteogenesis-related genes, including a 2.7-fold increase in expression of the key osteogenesis regulatory gene cbfa1, compared to untreated controls. In addition, exposure to PEMFs significantly increased ALP expression during the early stages of osteogenesis and substantially enhanced mineralization near the midpoint of osteogenesis. These results suggest that PEMFs enhance early cell proliferation in BMMSC-mediated osteogenesis, and accelerate the osteogenesis.
骨生成是一系列复杂的事件，涉及间充质干细胞分化以生成新骨。在本研究中，我们检测了脉冲电磁场（PEMFs）对骨髓间充质干细胞（BMMSCs）成骨分化过程中细胞增殖、碱性磷酸酶（ALP）活性、细胞外基质矿化及基因表达的影响。与未处理细胞相比，在分化第1天，BMMSCs暴露于PEMFs使细胞增殖增加了29.6%。半定量逆转录聚合酶链反应（RT-PCR）表明，PEMFs显著改变了成骨相关基因的时间表达，与未处理对照相比，关键成骨调节基因cbfa1的表达增加了2.7倍。此外，暴露于PEMFs在成骨早期显著增加了ALP表达，并在成骨中期附近显著增强了矿化。这些结果表明，PEMFs增强了BMMSC介导的成骨过程中的早期细胞增殖，并加速了成骨。
