Laboratories of The Rockefeller Institute for Medical Research.
J Exp Med. 1928 Jan 1;47(1):171-81. doi: 10.1084/jem.47.1.171.
Attempts to confirm certain statements that ether-soluble specific substances can be obtained from B. typhosus have lead to negative results. Two serologically active protein substances and another that was non-protein have been separated from B. typhosus. The first two are not resistant to tryptic digestion or to treatment with alkaline hypochlorite solution whereas the third resists both. One of the proteins could be extracted with 75 per cent alcohol. Specific precipitable substances reacting like the non-protein substance of B. typhosus and containing large amounts of carbohydrates have been prepared from B. paratyphosus B, B. enteritidis, and Proteus HX19 and OX19. Observations on the serological behaviour of these preparations are described.
从 B 型伤寒杆菌中提取乙醚可溶性特定物质的尝试已得到否定的结果。已从 B 型伤寒杆菌中分离出两种血清学上具有活性的蛋白质物质和另一种非蛋白质物质。前两种物质对胰蛋白酶消化或碱性次氯酸盐溶液处理均不具有抗性，而第三种物质则对两者均具有抗性。其中一种蛋白质可以用 75%的酒精提取。已从 B 型副伤寒杆菌 B、肠炎沙门氏菌和变形杆菌 HX19 和 OX19 中制备出类似于 B 型伤寒杆菌非蛋白质物质且含有大量碳水化合物的可沉淀的特异性物质。对这些制剂的血清学行为的观察结果进行了描述。
