Department of Pathology, School of Medicine and Dentistry, University of Rochester, Rochester, N. Y.
J Exp Med. 1930 Mar 31;51(4):549-70. doi: 10.1084/jem.51.4.549.
Vital staining reactions of brilliant vital red and Niagara sky blue are studied in dogs and in rabbits. Either dye alone is taken up to form red or blue granules within the cytoplasm of macrophages and of certain other cell types. When the two dyes are injected simultaneously into the blood stream one finds that these cells build up granules which are purple from admixture of the two dyes. When several daily injections of one dye are followed by several daily injections of the other, one finds blue granules and red granules side by side within the cells, but no purple granules are found. This is thought to indicate that the dye is deposited in small foci which are active in a rather transitory way, and that the color of the granule is determined during its formative stage by the type of dye present in the fluids about the cell. The enlargement of phagocytic cells and the increase in their number with large dosage, or with repeated offerings of the dye, represents a method by which the cells maintain their phagocytic powers at the normal level. Evidence is offered to indicate that these and perhaps other compensatory changes may take place with great rapidity, so that it has been impossible to "block" or even reduce noticeably the ability of these cells to take up additional quantities of dye. Certain pitfalls in the experimental study of "blockade" are pointed out.
在狗和兔子身上研究了亮 vital 红和尼亚加拉天空蓝的 vital 染色反应。单独使用任一染料都会在巨噬细胞和某些其他细胞类型的细胞质内形成红色或蓝色颗粒。当两种染料同时注入血流中时，会发现这些细胞会形成紫色颗粒，这是两种染料混合的结果。当每天注射一种染料数次，然后再每天注射另一种染料数次时，会发现细胞内同时存在蓝色和红色颗粒，但没有发现紫色颗粒。这被认为表明染料沉积在活跃但短暂的小焦点中，并且颗粒的颜色在其形成阶段由细胞周围液体中存在的染料类型决定。吞噬细胞的增大及其数量的增加与大剂量或反复提供染料有关，这代表了细胞维持其正常吞噬能力的一种方法。有证据表明，这些甚至其他代偿性变化可能发生得非常迅速，因此我们不可能“阻断”甚至明显降低这些细胞摄取额外染料的能力。文中指出了“阻断”实验研究中的某些陷阱。
