Department of Pathology, School of Medicine and Dentistry, University of Rochester, Rochester, N. Y.
J Exp Med. 1930 Mar 31;51(4):531-48. doi: 10.1084/jem.51.4.531.
When brilliant vital red is injected into the blood stream of dogs much of it is slowly taken up into numerous phagocytes scattered throughout the tissues ("reticulo-endothelial system" of Aschoff). The rate at which the dye leaves the blood stream is determined in large part by the action of these phagocytic cells, but the excretion of dye into the bile is also in part responsible for the loss of dye from the plasma. The injection of a small amount of India ink into the blood stream results in lowering the rate at which the dye disappears from circulation. The fact that much of the carbon of the ink is promptly taken up by the phagocytes would lead one to suspect that they were saturated with foreign materials, or "blocked" against the entrance of dye, but it is shown that the ink causes a remarkable inhibition of the excretion of dye into the bile, and this alone seems to account for the longer retention of dye in the blood stream. There is no evidence that any of the retention is due to defective activity on the part of the phagocytes. Thus, prolonged retention of foreign materials in the blood stream cannot be cited to prove "blockade of the reticulo-endothelial system" unless one can rule out such peculiar reactions on the part of excretory organs. It is felt that the literature of "blockade" should be studied with such sources of error in mind. Preliminary studies indicate that the suppression of dye excretion by the liver is not due to the carbon content of the ink. Studies of other components of the ink are now in progress.
当将鲜艳的生命红注入狗的血流中时，其中大部分会被缓慢地吸收到散布在组织中的众多吞噬细胞中（阿肖夫的“网状内皮系统”）。染料从血流中离开的速度在很大程度上取决于这些吞噬细胞的作用，但染料排入胆汁也部分负责从血浆中损失染料。向血流中注射少量的印度墨水会导致染料从循环中消失的速度降低。墨水的碳元素很快被吞噬细胞吸收这一事实会使人怀疑它们已经被外来物质饱和，或者“阻止”了染料的进入，但事实证明，墨水会显著抑制染料排入胆汁，这似乎是染料在血液中滞留时间更长的唯一原因。没有证据表明任何保留是由于吞噬细胞的活性缺陷所致。因此，除非能够排除排泄器官的这种特殊反应，否则不能将外来物质在血液中长时间滞留作为“网状内皮系统阻塞”的证据。人们认为，在研究“阻塞”的文献时，应考虑到这些错误来源。初步研究表明，肝脏对染料排泄的抑制不是由于墨水的碳含量所致。目前正在对墨水的其他成分进行研究。
