溴隐亭对人类精子活力及其穿透宫颈黏液能力的影响。

The effect of bromocriptine on the motility of human spermatozoa and its capacity to penetrate the cervical mucus.

作者信息

Rojas F J, Djannati E, Rojas I M

机构信息

Department of Obstetrics and Gynecology, University of California Irvine Medical Center, Orange 92668.

出版信息

Fertil Steril. 1991 Jan;55(1):48-52. doi: 10.1016/s0015-0282(16)54057-6.

DOI:10.1016/s0015-0282(16)54057-6

PMID:1986972

Abstract

We investigated the direct effects of bromocriptine on the motility of the human ejaculated spermatozoa and its capacity to penetrate the cervical mucus (CM) in vitro. Washed sperm were incubated with a wide range of bromocriptine concentrations (0.005 to 5 mM). Progressive and total motility was evaluated after 15, 30, 60, and 180 minutes. No reduction of motility was observed at any concentration tested. Similar results were observed with semen samples. Failure to alter sperm motility was evident in samples with either good or reduced motility. Also, CM penetration as measured after 90 minutes by Penetrak assay (Serono Diagnostics, Randolph, MA) was not impaired by a concentration as high as 5 mM bromocriptine. We conclude that bromocriptine in a wide range of concentrations does not inhibit sperm motility nor does it impair the capability of the sperm to penetrate the CM. These data support the therapeutic use of daily vaginal bromocriptine for the treatment of infertility.

摘要

我们研究了溴隐亭对人类射出精子活力及其体外穿透宫颈黏液（CM）能力的直接影响。将洗涤后的精子与一系列不同浓度的溴隐亭（0.005至5 mM）一起孵育。在15、30、60和180分钟后评估精子的前向运动和总活力。在任何测试浓度下均未观察到活力降低。精液样本也得到了类似结果。在活力良好或活力降低的样本中，均明显未改变精子活力。此外，通过Penetrak检测法（Serono Diagnostics， Randolph，MA）在90分钟后测量的CM穿透能力，也未受到高达5 mM溴隐亭浓度的损害。我们得出结论，一系列浓度的溴隐亭既不抑制精子活力，也不损害精子穿透CM的能力。这些数据支持每日阴道使用溴隐亭治疗不孕症。