Department of Pathology, Harvard University Medical School, Boston.
J Exp Med. 1931 Apr 30;53(5):647-60. doi: 10.1084/jem.53.5.647.
India ink or graphite partides injected into an area of inflammation fail to disseminate to the tributary lymph nodes. When injected into a normal peritoneal cavity they rapidly appear in the retrosternal lymph nodes. When injected into an inflamed peritoneal cavity they are fixed in situ and fail to reach the regional lymph nodes. Graphite particles injected in the circulating blood stream enter an inflamed area both as free particles owing to increased capillary permeability and also as phagocyted material within leucocytes. Bacteria (B. prodigiosus) injected into inflamed tissue are fixed at the site of inflammation and fail to disseminate to the regional lymph nodes as readily as when injected into normal tissue. Bacteria (B. prodigiosus) injected at the periphery of an inflamed area do not readily penetrate into the site of inflammation. The experiments furnish evidence, in addition to that already provided, that fixation of foreign substances by the inflammatory reaction is primarily due to mechanical obstruction caused by a fibrin network and by thrombosed lymphatics at the site of inflammation. Bacteria (B. prodigiosus and B. pyocyaneus) injected intravenously rapidly enter an inflamed area. It is suggested that localization of bacteria in a locus minoris resistentiae may be explained as the result of increased capillary permeability with subsequent accumulation and fixation of bacteria from the blood stream at the point of injury.
注入炎症区域的印度墨水或石墨颗粒不会扩散到支流淋巴结。当注入正常腹腔时，它们会迅速出现在胸骨后淋巴结中。当注入发炎的腹腔时，它们会固定在原地，无法到达区域淋巴结。注入循环血液中的石墨颗粒会以游离颗粒的形式（由于毛细血管通透性增加）和白细胞内吞噬的物质进入炎症区域。注入炎症组织的细菌（B. prodigiosus）会固定在炎症部位，并且不像注入正常组织那样容易扩散到区域淋巴结。注入炎症区域边缘的细菌不容易渗透到炎症部位。这些实验除了已经提供的证据外，还提供了证据表明，炎症反应对异物的固定主要是由于炎症部位的纤维蛋白网络和血栓形成的淋巴管造成的机械阻塞所致。静脉内注射的细菌（B. prodigiosus 和 B. pyocyaneus）会迅速进入炎症区域。有人认为，细菌在抵抗力较弱的部位定位可能是由于毛细血管通透性增加，随后在受伤部位从血液中积累和固定细菌所致。
