组织培养研究细菌过敏反应：二、C 群溶血性链球菌感染豚鼠组织的反应。
TISSUE CULTURE STUDIES ON BACTERIAL HYPERSENSITIVITY : II. REACTIONS OF TISSUES FROM GUINEA PIGS INFECTED WITH GROUP C HEMOLYTIC STREPTOCOCCI.
机构信息
Hospital of The Rockefeller Institute for Medical Research.
出版信息
J Exp Med. 1936 Aug 31;64(3):355-68. doi: 10.1084/jem.64.3.355.
Abstract
- Guinea pigs infected with naturally pathogenic hemolytic streptococci (group C-Lancefield) develop a low grade chronic type of disease characterized chiefly by purulent lymphadenitis. 2. Cutaneous hyperreactivity to a crude streptococcal extract invariably occurred during the course of this infection. 3. Production of antibodies (precipitins and agglutinins) was studied. 4. The hemolytic streptococcal extract had a specific toxic effect, when tested in vitro, on cells from infected animals; this was shown by microscopic evidence of cellular injury, and by quantitative inhibition of cellular migration and growth. The specificity of the reaction was proven by testing with other cytotoxic substances. 5. There was no parallelism between skin hypersensitivity and humoral antibody titer. 6. There was no correlation between the degree of skin reactivity to the bacterial extract and the degree of sensitivity of splenic cells to the toxic action of the same extract in vitro. 7. Comparison of cellular sensitivity to tuberculin with cellular sensitivity to streptococcal extract in cultures of guinea pig tissues showed that the former was more intense and was more persistent on prolonged growth in vitro.
摘要
感染天然致病性溶血性链球菌（C-Lancefield 组）的豚鼠会发展出一种低度慢性疾病，其特征主要为脓性淋巴结炎。
在感染过程中，豚鼠对粗制链球菌提取物的皮肤超敏反应始终发生。
研究了抗体（沉淀素和凝集素）的产生。
溶血性链球菌提取物在体外对感染动物的细胞具有特异性毒性作用；这通过细胞损伤的显微镜证据以及细胞迁移和生长的定量抑制得到证明。通过用其他细胞毒性物质进行测试，证明了反应的特异性。
皮肤过敏反应与体液抗体滴度之间没有平行关系。
皮肤对细菌提取物的反应程度与脾细胞对同一提取物在体外的毒性作用的敏感性之间没有相关性。
与体外长期生长相比，豚鼠组织培养中细胞对结核菌素的敏感性与细胞对链球菌提取物的敏感性比较表明，前者更强烈且更持久。
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