来自棒状链霉菌的β-内酰胺羟化酶基因（cefF）的克隆与测序：基因复制可能导致放线菌中羟化酶和扩环酶活性的分离。
Cloning and sequencing of the beta-lactam hydroxylase gene (cefF) from Streptomyces clavuligerus: gene duplication may have led to separate hydroxylase and expandase activities in the actinomycetes.
作者信息
Kovacevic S, Miller J R
机构信息
Department of Molecular Genetics Research, Lilly Research Laboratories, Lilly Corporate Center, Indianapolis, Indiana 46285.
出版信息
J Bacteriol. 1991 Jan;173(1):398-400. doi: 10.1128/jb.173.1.398-400.1991.
Abstract
The deacetylcephalosporin C synthetase (hydroxylase) gene from Streptomyces clavuligerus has been cloned and sequenced. The open reading frame codes for a protein with an Mr of 34,584. The hydroxylase gene (cefF) is closely linked to the epimerase gene (cefD) and the expandase gene (cefE) and is transcribed in the opposite orientation. The hydroxylase and expandase genes are 59 and 71% identical at the amino acid and DNA levels, respectively. cefE and cefF may have arisen from a gene duplication in the actinomycetes.
摘要
来自棒状链霉菌的脱乙酰头孢菌素C合成酶（羟化酶）基因已被克隆和测序。开放阅读框编码一种分子量为34,584的蛋白质。羟化酶基因（cefF）与差向异构酶基因（cefD）和扩展酶基因（cefE）紧密相连，并以相反的方向转录。羟化酶基因和扩展酶基因在氨基酸水平和DNA水平上分别有59%和71%的同源性。cefE和cefF可能起源于放线菌中的基因复制。
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