Smith D J, Burnham M K, Bull J H, Hodgson J E, Ward J M, Browne P, Brown J, Barton B, Earl A J, Turner G
Department of Microbiology, University of Bristol, UK.
EMBO J. 1990 Mar;9(3):741-7. doi: 10.1002/j.1460-2075.1990.tb08168.x.
A cosmid clone containing closely linked beta-lactam antibiotic biosynthetic genes was isolated from a gene library of Flavobacterium sp. SC 12,154. The location within the cluster of the DNA thought to contain the gene for delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase (ACVS), the first step in the beta-lactam antibiotic biosynthetic pathway, was identified by a novel method. This DNA facilitated the isolation, by cross-hybridization, of the corresponding DNA from Streptomyces clavuligerus ATCC 27064, Penicillium chrysogenum Oli13 and Aspergillus nidulans R153. Evidence was obtained which confirmed that the cross-hybridizing sequences contained the ACVS gene. In each case the ACVS gene was found to be closely linked to other beta-lactam biosynthetic genes and constituted part of a gene cluster.
从黄杆菌属SC 12154的基因文库中分离出一个含有紧密连锁的β-内酰胺抗生素生物合成基因的黏粒克隆。通过一种新方法确定了DNA簇中被认为含有δ-（L-α-氨基己二酰基）-L-半胱氨酰-D-缬氨酸合成酶（ACVS）基因的位置，ACVS是β-内酰胺抗生素生物合成途径的第一步。该DNA通过交叉杂交促进了从棒状链霉菌ATCC 27064、产黄青霉Oli13和构巢曲霉R153中分离出相应的DNA。获得的证据证实了交叉杂交序列包含ACVS基因。在每种情况下，都发现ACVS基因与其他β-内酰胺生物合成基因紧密连锁，并构成基因簇的一部分。
