β-内酰胺抗生素生物合成基因在原核生物和真核生物中以基因簇的形式保守存在。
Beta-lactam antibiotic biosynthetic genes have been conserved in clusters in prokaryotes and eukaryotes.
作者信息
Smith D J, Burnham M K, Bull J H, Hodgson J E, Ward J M, Browne P, Brown J, Barton B, Earl A J, Turner G
机构信息
Department of Microbiology, University of Bristol, UK.
出版信息
EMBO J. 1990 Mar;9(3):741-7. doi: 10.1002/j.1460-2075.1990.tb08168.x.
A cosmid clone containing closely linked beta-lactam antibiotic biosynthetic genes was isolated from a gene library of Flavobacterium sp. SC 12,154. The location within the cluster of the DNA thought to contain the gene for delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase (ACVS), the first step in the beta-lactam antibiotic biosynthetic pathway, was identified by a novel method. This DNA facilitated the isolation, by cross-hybridization, of the corresponding DNA from Streptomyces clavuligerus ATCC 27064, Penicillium chrysogenum Oli13 and Aspergillus nidulans R153. Evidence was obtained which confirmed that the cross-hybridizing sequences contained the ACVS gene. In each case the ACVS gene was found to be closely linked to other beta-lactam biosynthetic genes and constituted part of a gene cluster.
从黄杆菌属SC 12154的基因文库中分离出一个含有紧密连锁的β-内酰胺抗生素生物合成基因的黏粒克隆。通过一种新方法确定了DNA簇中被认为含有δ-（L-α-氨基己二酰基）-L-半胱氨酰-D-缬氨酸合成酶（ACVS）基因的位置，ACVS是β-内酰胺抗生素生物合成途径的第一步。该DNA通过交叉杂交促进了从棒状链霉菌ATCC 27064、产黄青霉Oli13和构巢曲霉R153中分离出相应的DNA。获得的证据证实了交叉杂交序列包含ACVS基因。在每种情况下，都发现ACVS基因与其他β-内酰胺生物合成基因紧密连锁，并构成基因簇的一部分。
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