Viscor Ginés, Javierre Casimiro, Pagès Teresa, Ventura Josep-Lluis, Ricart Antoni, Martin-Henao Gregorio, Azqueta Carmen, Segura Ramon
Departament de Fisiologia-Biologia, Universitat de Barcelona, Av. Diagonal 645, E-08028 Barcelona, Spain.
J Transl Med. 2009 Oct 29;7:91. doi: 10.1186/1479-5876-7-91.
Our goal was to determine whether short-term intermittent hypoxia exposure, at a level well tolerated by healthy humans and previously shown by our group to increase EPO and erythropoiesis, could mobilize hematopoietic stem cells (HSC) and increase their presence in peripheral circulation.
Four healthy male subjects were subjected to three different protocols: one with only a hypoxic stimulus (OH), another with a hypoxic stimulus plus muscle electrostimulation (HME) and the third with only muscle electrostimulation (OME). Intermittent hypobaric hypoxia exposure consisted of only three sessions of three hours at barometric pressure 540 hPa (equivalent to an altitude of 5000 m) for three consecutive days, whereas muscular electrostimulation was performed in two separate periods of 25 min in each session. Blood samples were obtained from an antecubital vein on three consecutive days immediately before the experiment and 24 h, 48 h, 4 days and 7 days after the last day of hypoxic exposure.
There was a clear increase in the number of circulating CD34+ cells after combined hypobaric hypoxia and muscular electrostimulation. This response was not observed after the isolated application of the same stimuli.
Our results open a new application field for hypobaric systems as a way to increase efficiency in peripheral HSC collection.
我们的目标是确定短期间歇性低氧暴露，在健康人可良好耐受且我们团队之前已证明可增加促红细胞生成素（EPO）和红细胞生成的水平下，是否能动员造血干细胞（HSC）并增加其在外周循环中的存在。
四名健康男性受试者接受三种不同方案：一种仅给予低氧刺激（OH），另一种给予低氧刺激加肌肉电刺激（HME），第三种仅给予肌肉电刺激（OME）。间歇性低压低氧暴露仅包括连续三天在气压540 hPa（相当于海拔5000米）下进行三次每次三小时的时段，而肌肉电刺激在每个时段分两个25分钟的单独时间段进行。在实验前连续三天以及低氧暴露最后一天后的24小时、48小时、4天和7天，从前臂静脉采集血样。
低压低氧和肌肉电刺激联合应用后，循环CD34 +细胞数量明显增加。单独应用相同刺激未观察到这种反应。
我们的结果为低压系统开辟了一个新的应用领域，作为提高外周造血干细胞采集效率的一种方法。
