National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Department of Health and Human Services, Bethesda, MD 20892, USA.
Curr Opin Chem Biol. 2009 Dec;13(5-6):687-96. doi: 10.1016/j.cbpa.2009.09.030. Epub 2009 Oct 29.
Genetic manipulation is an important facet of synthetic biology but can be complicated by undesired nuclease degradation. Incorporating non-natural nucleic acids into a gene could convey resistance to nucleases and promote expression. The compatibility of non-natural nucleosides with polymerases is reviewed with a focus on results from the past two years. Details are provided about how the different systems could be useful in synthetic biology.
遗传操作是合成生物学的一个重要方面,但可能会因非预期的核酸酶降解而变得复杂。将非天然核酸掺入基因中可以赋予对核酸酶的抗性并促进表达。本文综述了非天然核苷与聚合酶的兼容性,重点介绍了过去两年的研究结果。详细介绍了不同系统在合成生物学中的潜在应用。