非天然核酸在合成生物学中的应用。
Non-natural nucleic acids for synthetic biology.
机构信息
National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Department of Health and Human Services, Bethesda, MD 20892, USA.
出版信息
Curr Opin Chem Biol. 2009 Dec;13(5-6):687-96. doi: 10.1016/j.cbpa.2009.09.030. Epub 2009 Oct 29.
Abstract
Genetic manipulation is an important facet of synthetic biology but can be complicated by undesired nuclease degradation. Incorporating non-natural nucleic acids into a gene could convey resistance to nucleases and promote expression. The compatibility of non-natural nucleosides with polymerases is reviewed with a focus on results from the past two years. Details are provided about how the different systems could be useful in synthetic biology.
摘要
遗传操作是合成生物学的一个重要方面，但可能会因非预期的核酸酶降解而变得复杂。将非天然核酸掺入基因中可以赋予对核酸酶的抗性并促进表达。本文综述了非天然核苷与聚合酶的兼容性，重点介绍了过去两年的研究结果。详细介绍了不同系统在合成生物学中的潜在应用。
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