MTech programme in Bioprocess Technology, Institute of Chemical Technology, Mumbai, Maharashtra, 400019, India.
DBT-ICT Centre for Energy Biosciences, Institute of Chemical Technology, Nathalal Parekh Marg, Matunga (East), Mumbai, Maharashtra, 400019, India.
Microb Cell Fact. 2022 May 28;21(1):100. doi: 10.1186/s12934-022-01828-y.
The surging demand of value-added products has steered the transition of laboratory microbes to microbial cell factories (MCFs) for facilitating production of large quantities of important native and non-native biomolecules. This shift has been possible through rewiring and optimizing different biosynthetic pathways in microbes by exercising frameworks of metabolic engineering and synthetic biology principles. Advances in genome and metabolic engineering have provided a fillip to create novel biomolecules and produce non-natural molecules with multitude of applications. To this end, numerous MCFs have been developed and employed for production of non-natural nucleic acids, proteins and different metabolites to meet various therapeutic, biotechnological and industrial applications. The present review describes recent advances in production of non-natural amino acids, nucleic acids, biofuel candidates and platform chemicals.
增值产品的需求不断增长，推动了实验室微生物向微生物细胞工厂（MCFs）的转变，以促进大量重要天然和非天然生物分子的生产。这一转变通过对微生物中的不同生物合成途径进行重新布线和优化，利用代谢工程和合成生物学原理的框架成为可能。基因组和代谢工程的进步为创造新型生物分子和生产具有多种应用的非天然分子提供了助力。为此，已经开发并使用了许多 MCF 来生产非天然核酸、蛋白质和不同代谢物，以满足各种治疗、生物技术和工业应用的需求。本综述描述了非天然氨基酸、核酸、生物燃料候选物和平台化学品生产方面的最新进展。
