Faculty of Health & Medical Sciences, University of Surrey, Guildford, Surrey, GU2 7XH, UK.
Cell Stress Chaperones. 2010 Jul;15(4):379-86. doi: 10.1007/s12192-009-0152-7. Epub 2009 Oct 31.
Heat shock protein 27 (Hsp27) is over-expressed when cells are exposed to stressful conditions that include oxidative stress. Oxidative stress has been implicated in the pathogenesis of cardiovascular disease (CVD), diabetes and insulin resistance. We have investigated the concentrations of serum Hsp27 antigen and antibodies in subjects from different glycaemic categories, who either did or did not have established CVD. Serum Hsp27 antigen and antibody levels (immunoglobulins M and G (IgM and IgG)) were determined by enzyme-linked immunosorbent assays (ELISAs) in 68 individuals: 26 with normal glucose tolerance (NGT), 10 with (+) and 16 without (-) a history of CVD and 42 individuals with varying degrees of glucose intolerance (GI; 21 with and 21 without a history of CVD). Insulin sensitivity was determined in each subject using indices derived from the homeostasis model assessment of sensitivity and the insulin sensitivity index for glycaemia. Serum Hsp27 concentrations were significantly higher in GI (+CVD) subjects compared to GI (-CVD) subjects (p = 0.03), NGT (-CVD) subjects (p = 0.02) and NGT (+CVD) subjects (p = 0.04) and were positively correlated to fasting plasma glucose for all subjects (r = 0.28, p = 0.03). IgM antibody levels were significantly higher in GI (+CVD) subjects compared to NGT (-CVD) group (p = 0.02) and were inversely related to fasting insulin concentrations (r = -0.27, p = 0.04) and the 2-h insulin concentrations (r = -0.29, p = 0.03) for all subjects. Serum IgG antibody levels were higher in GI (+CVD) group compared to GI (-CVD) group (p = 0.06). In conclusion, Hsp27 and its antibody concentrations appear to relate to the presence of cardiovascular complications in patients with GI.
热休克蛋白 27（Hsp27）在细胞暴露于应激条件下（包括氧化应激）时过度表达。氧化应激与心血管疾病（CVD）、糖尿病和胰岛素抵抗的发病机制有关。我们研究了不同血糖类别受试者的血清 Hsp27 抗原和抗体浓度，这些受试者要么有，要么没有已确立的 CVD。通过酶联免疫吸附试验（ELISA）在 68 名个体中确定了血清 Hsp27 抗原和抗体水平（免疫球蛋白 M 和 G（IgM 和 IgG））：26 名具有正常葡萄糖耐量（NGT），10 名有（+）和 16 名没有（-）CVD 病史，42 名具有不同程度的葡萄糖耐量（GI；21 名有和 21 名没有 CVD 病史）。使用稳态模型评估敏感性和血糖胰岛素敏感性指数得出的指数，在每个受试者中确定胰岛素敏感性。与 GI（-CVD）受试者（p = 0.02）和 NGT（+CVD）受试者（p = 0.04）相比，GI（+CVD）受试者的血清 Hsp27 浓度显著升高，与所有受试者的空腹血糖呈正相关（r = 0.28，p = 0.03）。与 NGT（-CVD）组相比，GI（+CVD）受试者的 IgM 抗体水平显著升高（p = 0.02），并且与空腹胰岛素浓度呈负相关（r = -0.27，p = 0.04）和所有受试者的 2 小时胰岛素浓度（r = -0.29，p = 0.03）。与 GI（-CVD）组相比，GI（+CVD）组的血清 IgG 抗体水平较高（p = 0.06）。总之，Hsp27 及其抗体浓度似乎与 GI 患者心血管并发症的存在有关。
