Med Fakultät, Universität Bern, Switzerland.
Injury. 2009 Nov;40 Suppl 4:S95-102. doi: 10.1016/j.injury.2009.10.043.
Regeneration of living tissue varies with species, age and type of tissue, and undoubtedly with the biological and mechanical environment of the precise tissue. Autologous cancellous bone grafting is a well-known technique that provides bony regeneration. We investigated the efficiency of autologous bone grafting in a well-vascularised muscle environment, and additionally when isolated from the muscle and connected only to the bony environment. We designed a reproducible animal model producing a stable 3cm middiaphyseal bone and periosteal defect on sheep femurs and created a foreign-body membrane with a temporary poly-methylmethacrylate spacer. The foreign-body membrane had the outer dimension of the removed bone segment. We then ascertained the bony regeneration potential within the bone defect using autologous cancellous bone graft. Regeneration of bone is enhanced considerably by an autologous foreign-body membrane that separates the interfragmentary space from the muscular environment. This effect is independent of the autologous bone graft. The results suggest that bone behaves like a compartment that protects its specific humoral or its cellular environment, or both. Regeneration of bone can be enhanced by compartmentalisation of the bone defect.
组织的再生能力因物种、年龄和组织类型而异，而且无疑还与特定组织的生物和机械环境有关。自体松质骨移植是一种众所周知的技术，可提供骨再生。我们研究了在血运丰富的肌肉环境中以及从肌肉中分离出来并仅与骨环境相连的情况下，自体骨移植的效率。我们设计了一种可重现的动物模型，在绵羊股骨上产生稳定的 3cm 骨干中段和骨膜缺损，并使用临时聚甲基丙烯酸甲酯间隔物形成异物膜。该异物膜具有切除骨段的外尺寸。然后，我们使用自体松质骨移植物确定骨缺损内的骨再生潜力。自体异物膜将骨碎片间的空间与肌肉环境隔开，大大增强了骨的再生。这种效果与自体骨移植物无关。结果表明，骨的行为类似于一个隔室，可保护其特定的体液或细胞环境，或两者兼而有之。通过对骨缺损进行分隔，可以增强骨的再生。
