Department of Orthopedic Surgery, The First Affiliated Hospital of Shandong First Medical University, Jinan, Shandong Province, 250014, People's Republic of China.
Department of Orthopedic Surgery, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, Shandong Province, 250014, People's Republic of China.
Int J Nanomedicine. 2023 Mar 21;18:1365-1380. doi: 10.2147/IJN.S397316. eCollection 2023.
The repair and treatment of infected bone defects (IBD) is a common challenge faced by orthopedic clinics, medical materials science, and tissue engineering.
Based on the treatment requirements of IBD, we utilized multidisciplinary knowledge from clinical medicine, medical materials science, and tissue engineering to construct a high-efficiency vancomycin sustained-release system with nanodiamond (ND) and prepare a composite scaffold. Its effect on IBD treatment was assessed from materials, cytology, bacteriology, and zoology perspectives.
The results demonstrated that the Van-ND-45S5 scaffold exhibited an excellent antibacterial effect, biocompatibility, and osteogenesis in vitro. Moreover, an efficient animal model of IBD was established, and a Van-ND-45S5 scaffold was implanted into the IBD. Radiographic and histological analyses and bone repair-related protein expression, confirmed that the Van-ND-45S5 scaffold had good biocompatibility and osteogenic and anti-infective activities in vivo.
Collectively, our findings support that the Van-ND-45S5 scaffold is a promising new material and approach for treating IBD with good antibacterial effects, biocompatibility, and osteogenesis.
感染性骨缺损（IBD）的修复和治疗是骨科临床、医学材料科学和组织工程面临的共同挑战。
根据 IBD 的治疗要求，我们利用临床医学、医学材料科学和组织工程的多学科知识，构建了一种高效的纳米金刚石（ND）万古霉素缓释系统，并制备了一种复合支架。从材料、细胞学、细菌学和动物学角度评估了其对 IBD 治疗的效果。
结果表明，Van-ND-45S5 支架在体外表现出优异的抗菌效果、生物相容性和成骨作用。此外，建立了高效的 IBD 动物模型，并将 Van-ND-45S5 支架植入 IBD 中。影像学和组织学分析以及与骨修复相关的蛋白表达证实，Van-ND-45S5 支架在体内具有良好的生物相容性、成骨和抗感染活性。
综上所述，我们的研究结果支持 Van-ND-45S5 支架是一种有前途的新材料和方法，可用于治疗具有良好抗菌效果、生物相容性和成骨作用的 IBD。
